Human pluripotent stem cells (hPSCs) are a useful cell source for regenerative medicine. Despite having a potential of hPSCs for cell-based therapy, there is a need for a selective human pluripotency sensor for monitoring of live hPSCs. Here, we report the discovery of a novel pluripotency sensor (SHI5) from BODIPY-based library by high-throughput cell-based screening and describe the use of SHI5 to identify and isolate human embryonic stem cells and human induced pluripotent stem cells. We demonstrate that SHI5-based assay can be applied to live cells that gain pluripotency in the reprogramming process without any effect on their viability. We also show that SHI5 is internalized through a clathrin-mediated endocytosis pathway. These findings suggest that SHI5 can be an attractive sensor for pluripotency cells during reprogramming. Taken together, SHI5-based screening for hPSCs opens probably unlimited possibilities of detection probe for hPSC therapy via assures their safety issue.

人多能干细胞（hPSC）是再生医学的有用细胞来源。尽管hPSC具有用于基于细胞的治疗的潜力，但是仍需要用于监测活hPSC的选择性人多能传感器。在这里，我们报告基于高通量的基于细胞的筛选从基于BODIPY的库中发现了一种新型的多能性传感器（SHI5），并描述了使用SHI5来识别和分离人胚胎干细胞和人诱导的多能干细胞。我们证明了基于SHI5的检测可以应用于在重编程过程中获得多能性的活细胞，而不会对其生存能力产生任何影响。我们还表明，SHI5是通过网格蛋白介导的内吞途径内化的。这些发现表明，SHI5可能是重编程过程中多能细胞的诱人传感器。在一起