This study was undertaken not only to evaluate detoxification impact vs. adaptation on ethanologenic fermentation but also to optimize the fermentation condition to produce ethanol from Sugarcane Bagasse Hydrolysate (SBH). Detoxification by over-liming significantly improved the fermentability of the Concentrated Sugarcane Bagasse Hydrolysate (CSBH) by a wild strain of Barnettozyma californica. Compared to non-detoxified CSBH, ethanol production by the wild type strain increased from 1.233 g L⁻¹ to 3.8031 g L⁻¹, after 32 h and 24 h fermentation, respectively. Detoxification of SBH and CSBH showed a reverse effect on the adapted yeast. In detoxified CSBH, fermentation time increased to 32 h (3.084 g L⁻¹) by adapted strain, while ethanol production was 3.803 g L⁻¹ after 24 h by the non-adapted strain. Also, it should be noted that the parental yeast's capacity was found to be practicable in SBH without a detoxification and adaptation process. The screening and optimization of process parameters and critical medium components were done using the Plackett–Burman and Box–Behnken experimental design, respectively. (NH₄)₂SO₄, (NH₄)₂PO_4, ZnCl₂, hydrolysate concentration, incubation temperature, and flask volume were found to be the main factors affecting ethanol production. Finally, a yield of 0.44 g g⁻¹ was obtained, which is almost twice as high as the non-optimized medium. Taken together, these results indicate that hydrolysate detoxification and yeast adaptation through adaptive evolution and medium optimization are effective methods to overcome the bottleneck of lignocellulosic ethanol production.

进行这项研究不仅是为了评估解毒作用与对产乙醇发酵的适应性，而且是为了优化发酵条件以从甘蔗渣水解液（SBH）生产乙醇。通过过量石灰解毒可以显着提高浓缩的Barnettozyma californica野生菌株对浓缩甘蔗蔗渣水解液（CSBH）的发酵能力。与未排毒的CSBH相比，在发酵32小时和24小时后，野生型菌株的乙醇产量分别从1.233 g L ^-1增加到3.8031 g L ^-1。SBH和CSBH的解毒对适应的酵母菌显示出相反的作用。在解毒的CSBH中，发酵时间增加到32 h（3.084 g L ^-1），而未适应菌株在24 h后的乙醇产量为3.803 g L ^-1。另外，应该指出的是，在没有排毒和适应过程的情况下，SBH中亲本酵母的能力是可行的。分别使用Plackett–Burman和Box–Behnken实验设计进行了工艺参数和关键介质成分的筛选和优化。发现（NH ₄）₂ SO ₄，（NH ₄）₂ PO _4， ZnCl ₂，水解产物浓度，温育温度和烧瓶容量是影响乙醇生产的主要因素。最后，产量为0.44 g g ^-1获得的碳几乎是未优化培养基的两倍。综上所述，这些结果表明水解产物的解毒和通过适应性进化和培养基优化的酵母适应是克服木质纤维素乙醇生产瓶颈的有效方法。