Abstract A bacterial consortium consisting five efficient polyaromatic hydrocarbon (PAH) degraders (Pseudomonas aeruginosa PDB1, Pseudomonas fragi DBC, Klebsiella pneumoniae AWD5, Alcaligenes faecalis BDB4 and Acinetobacter sp. PDB4) was bioaugmented in pyrene contaminated soil through Jatropha curcas rhizosphere, to understand its effect on changes in bacterial community structure during the process of rhizosphere mediated biodegradation of pyrene. This consortium was highly effective in rhizosphere mediated remediation, as 97.2% of pyrene was degraded within 60 days. The better degradation of pyrene in soil was correlated with the changes in bacterial community structure in rhizosphere of J. curcas, for different trials. The Proteobacteria remained the most abundant group among different treatments, but its abundance was 30.7% higher in the rhizosphere of plants growing in pyrene contaminated soil augmented with consortium. Sphingomonas, Sphingobium, Achromobacter, Ralstonia, Pseudoxanthomonas, Devosia, Cupriavidus, and the members of Sphingomonadeceae, Rhizobiaceae, Xanthomonadeceae, were dominant within Proteobacteria, where pyrene degradation was maximum. There was strong correlation between abundances of group of bacteria which were being affected with pyrene contamination or consortium treatment, and this effect was spread over for a group of bacteria, whose abundances were interdependent. Consequently, the treatment of PAH degrading consortium restored and recruited group of bacteria, which were otherwise depleted in presence of pyrene. Therefore, the application of consortium not only facilitated the degradation of pyrene, but also resulted in modulation of the bacterial community in the process of rhizosphere mediated biodegradation of pyrene in soil.

中文翻译：

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麻风树根际细菌群落与多环芳烃降解菌群对芘生物降解有关

摘要 由五种高效多环芳烃 (PAH) 降解菌（铜绿假单胞菌 PDB1、脆弱假单胞菌 DBC、肺炎克雷伯菌 AWD5、粪产碱菌 BDB4 和不动杆菌属 PDB4）组成的细菌聚生体在芘污染土壤中进行生物强化，通过麻风树对其影响在根际介导的芘生物降解过程中细菌群落结构的变化。该联盟在根际介导的修复中非常有效，因为 97.2% 的芘在 60 天内降解。对于不同的试验，土壤中芘的更好降解与麻疯树根际细菌群落结构的变化相关。Proteobacteria 仍然是不同处理中最丰富的组，但其丰度为 30。在芘污染土壤中生长的植物根际高出 7%。Sphingomonas、Sphingobium、Achromobacter、Ralstonia、Pseudoxanthomonas、Devosia、Cupriavidus 以及鞘单胞菌科、根瘤菌科、黄单胞菌科的成员在 Proteobacteria 中占优势，其中芘降解最大。受芘污染或聚生体处理影响的细菌群的丰度之间存在很强的相关性，这种影响在一群细菌中传播，其丰度是相互依赖的。因此，PAH 降解聚生体的处理恢复并招募了一组细菌，否则这些细菌在芘存在时会被耗尽。因此，联合体的应用不仅促进了芘的降解，