Soshiho-tang (SSHT) has traditionally been used to treat gastrointestinal disorders. In this experiment, we investigated the protective effect of SSHT on inflammatory liver injury in lipopolysaccharide (LPS)-sensitized mice. Male C57BL/6J mice aged 6 weeks were randomly placed in 6 groups (n = 5): normal mice (CTR), LPS-sensitized mice (LPS), LPS-sensitized mice treated with dexamethasone (DEX) and LPS-sensitized mice treated with 0.05, 0.55, and 5.55 g/kg of SSHT (SSHT 0.05, SSHT 0.55, and SSHT 5.55). Various doses of SSHT was given once a day for 7 days. After 2 h of LPS injection, the liver tissue was collected. SSHT pretreatment recovered hemorrhage of liver tissues in LPS-induced acute liver injury. The expressions of MAP Kinase, NF-κB, IκBα, p-IκBα, COX-2, and iNOS protein levels were markedly decreased by SSHT-treated liver tissues. Additionally, SSHT pretreatment significantly regulated the expressions of MCP-1, TNF-α, and IL-6 cytokines. These results suggest the potential of SSHT on the protection of acute liver injury.

中文翻译：

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Soshiho-tang通过减轻炎症反应来保护LPS诱导的急性肝损伤。

传统上，Soshiho-tang（SSHT）用于治疗胃肠道疾病。在本实验中，我们研究了SSHT对脂多糖（LPS）致敏小鼠的炎症性肝损伤的保护作用。将6周龄的雄性C57BL / 6J小鼠随机分为6组（n = 5）：正常小鼠（CTR），LPS致敏小鼠（LPS），地塞米松（DEX）处理的LPS致敏小鼠和0.05、0.55和5.55 g / kg SSHT处理的LPS致敏小鼠（SSHT 0.05， SSHT 0.55和SSHT 5.55）。每天一次给予不同剂量的SSHT，共7天。LPS注射2小时后，收集肝脏组织。SSHT预处理可恢复LPS诱发的急性肝损伤中肝脏组织的出血。SSHT处理的肝组织显着降低了MAP激酶，NF-κB，IκBα，p-IκBα，COX-2和iNOS蛋白的表达。此外，SSHT预处理可显着调节MCP-1，TNF-α和IL-6细胞因子的表达。这些结果表明SSHT在保护急性肝损伤中的潜力。