Golgi staining allows for the analysis of neuronal arborisations and connections and is considered a powerful tool in basic and clinical neuroscience. The fundamental rules for improving neuronal staining using the Golgi-Cox method are not fully understood; both intrinsic and extrinsic factors may control the staining process. Therefore, various conditions were tested to improve the Golgi-Cox protocol for vibratome-cut rat brain sections. Optimal staining of cortical neurons was achieved after 72 h of impregnation. Well-stained neurons in both cortical and subcortical structures were observed after 96 h of impregnation. The dendritic arborisation pattern of cortical neurons derived from the 72-h impregnation group was comparable to those of the 96 and 168-h impregnation groups. The entire brain was stained well when the pH of the Golgi-Cox solution was 6.5 and that of the sodium carbonate solution was 11.2. Lack of brain perfusion or perfusion with 0.9% NaCl did not influence optimal neuronal staining. Perfusion with 37% formaldehyde, followed by impregnation, only resulted in glial staining, but perfusion with 4% formaldehyde facilitated both glial and neuronal staining. Whole brains required longer impregnation times for better staining. Although every factor had a role in determining optimal neuronal staining, impregnation time and the pH of staining solutions were key factors among them. This modified Golgi-Cox protocol provides a simple and economical procedure to stain both neurons and glia separately.

高尔基体染色允许分析神经元分支和连接，被认为是基础和临床神经科学的强大工具。使用Golgi-Cox方法改善神经元染色的基本规则尚不完全清楚；内在和外在因素都可以控制染色过程。因此，测试了各种条件以改善Golgi-Coxvibratome-cut 大鼠脑切片的协议。在浸渍 72 小时后实现了皮质神经元的最佳染色。在浸渍 96 小时后观察到皮层和皮层下结构中染色良好的神经元。来自 72 小时浸渍组的皮层神经元的树突分枝模式与 96 小时和 168 小时浸渍组的相似。当Golgi-Cox的 pH 值升高时，整个大脑都被很好地染色溶液为 6.5，碳酸钠溶液为 11.2。缺乏脑灌注或灌注 0.9% NaCl 不会影响最佳神经元染色。用 37% 甲醛灌注，然后浸渍，仅导致神经胶质染色，但用 4% 甲醛灌注促进神经胶质和神经元染色。整个大脑需要更长的浸渍时间以获得更好的染色。尽管每个因素都在决定最佳神经元染色中起作用，但浸渍时间和染色溶液的 pH 值是其中的关键因素。这种修改后的高尔基-考克斯协议提供了一个简单而经济的程序来分别染色神经元和神经胶质。