We are developing magnetic nanoparticle (NP) methods to characterize inflammation and infection in vivo . Peritoneal infection in C57BL/6 mice was used as a biological model. An intraperitoneal NP injection was followed by measurement of magnetic nanoparticle spectroscopy of Brownian rotation (MSB) spectra taken over time. MSB measures the magnetization of NPs in a low frequency alternating magnetic field. Two groups of three mice were studied; each group had two infected mice and one control with no infection. The raw MSB signal was compared with two derived metrics: the NP relaxation time and number of NPs present in the sensitive volume of the receive coil. A four compartment dynamic model was used to relate those physical properties to the relevant biological processes including phagocytic activity and migration. The relaxation time increased over time for all of the mice as the NPs were absorbed. The NP number decreased over time as the NPs were cleared from the sensi...

中文翻译：

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使用磁性纳米粒子光谱识别体内炎症

我们正在开发磁性纳米颗粒（NP）方法来表征体内炎症和感染。C57BL / 6小鼠的腹膜感染被用作生物学模型。腹膜内注射NP，随后测量随时间变化的布朗旋转（MSB）光谱的磁性纳米粒子光谱。MSB测量低频交变磁场中NP的磁化强度。研究了两组，每组三只小鼠。每组有两只被感染的小鼠和一个没有感染的对照。将原始MSB信号与两个导出的指标进行比较：NP弛豫时间和接收线圈敏感体积中存在的NP数量。使用四室动态模型将那些物理特性与相关的生物学过程相关，包括吞噬活性和迁移。对于所有小鼠，随着NP的吸收，弛豫时间随时间增加。NP数量随着时间的流逝而减少，因为从感觉中清除了NP。