As cell engineering technology advances, more complex synthetically designed cells and metabolically engineered cells are being developed. Engineered cells are important resources in industry. Similar to image watermarking, engineered cells should be watermarked for protection against improper use. In this study, a DNA steganography methodology was developed to hide messages in variable regions (single nucleotide polymorphisms) of the genome to create hidden messages and thereby prevent from hacking. Additionally, to detect errors (mutations) within the encrypted messages, a block sum check algorithm was employed, similar to that used in network data transmission to detect noise-induced information changes. This DNA steganography methodology could be used to hide secret messages in a genome and detect errors within the encrypted messages. This approach is expected to be useful for tracking cells and protecting biological assets (e.g., engineered cells).

中文翻译：

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DNA隐写术：将无法检测到的秘密信息隐藏在基因组的单核苷酸多态性内，并检测由突变引起的错误。

随着细胞工程技术的发展，正在开发更复杂的合成设计的细胞和代谢工程的细胞。工程电池是工业中的重要资源。与图像加水印相似，工程细胞应加水印以防止不当使用。在这项研究中，开发了一种DNA隐写术方法，以将消息隐藏在基因组的可变区域（单核苷酸多态性）中，以创建隐藏的消息，从而防止被黑。另外，为了检测加密消息中的错误（变异），采用了块和校验算法，类似于在网络数据传输中用于检测噪声引起的信息变化的算法。这种DNA隐写术方法学可用于隐藏基因组中的秘密消息，并检测加密消息中的错误。