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Boronic acid-functionalized mesoporous magnetic particles with a hydrophilic surface for the multimodal enrichment of glycopeptides for glycoproteomics.
Analyst ( IF 3.6 ) Pub Date : 2020-06-11 , DOI: 10.1039/d0an00648c
Lujie Yang 1 , Quanqing Zhang , Yuanyu Huang , Ling Lin , Hartmut Schlüter , Ke Wang , Cuiping Zhang , Pengyuan Yang , Hongxiu Yu
Affiliation  

Glycosylation is an important mechanism of secondary protein processing. Large-scale profiling of glycopeptides released by proteolytic digestion of glycoproteins from biologic samples with complex compositions is limited due to their low abundance. Herein, we present a multimodal material based on boronic acid-modified mesoporous magnetic particles with a hydrophilic surface and enlarged pores around 10 nm. Multimodal enrichment successfully improved the enrichment specificity and efficiency of BMMP by synergistic interaction of hydrophilicity and boronic acid functional groups. The 10 nm pore size allows glycopeptides to enter the channel. Hydrophilic glycopeptides could be selectively enriched with an extremely low limit of detection (0.33 fmol per μL) and a high selectivity (1 : 100). From 2 μL of human serum, 328 unique glycopeptides from 101 glycoproteins were identified. A total of 33% of those glycoproteins overlapped with FDA-cleared blood serum biomarkers. It is expected that BMMP in the future can be used for large-scale biomedical glycoproteomics studies.

中文翻译:

具有亲水性表面的硼酸官能化介孔磁性颗粒,用于糖蛋白组学中糖肽的多峰富集。

糖基化是二级蛋白质加工的重要机制。通过蛋白水解消化具有复杂成分的生物样品中糖蛋白而释放出的糖肽的大规模谱图分析由于其丰度低而受到限制。在本文中,我们提出了一种基于硼酸改性的介孔磁性颗粒的多峰材料,该材料具有亲水性表面和约10 nm的大孔。多峰富集通过亲水性和硼酸官能团的协同相互作用成功提高了BMMP的富集特异性和效率。10 nm的孔径允许糖肽进入通道。亲水糖肽可以选择性地富集,具有极低的检测限(0.33 fmol /μL)和高选择性(1:100)。从2μL人血清中 从101种糖蛋白中鉴定出328种独特的糖肽。这些糖蛋白中总共有33%与FDA清除的血清生物标志物重叠。预计将来BMMP可用于大规模的生物医学糖蛋白组学研究。
更新日期:2020-07-30
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