There is a growing need for biomaterial scaffolds that support engineering of soft tissue substitutes featuring structure and mechanical properties similar to those of the native tissue. This work introduces a new biomaterial system that is based on centimeter-long collagen fibers extracted from Sarcophyton soft corals, wrapped around frames to create aligned fiber arrays. The collagen arrays displayed hyperelastic and viscoelastic mechanical properties that resembled those of collagenous-rich tissues. Cytotoxicity tests demonstrated that the collagen arrays were nontoxic to fibroblast cells. In addition, fibroblast cells seeded on the collagen arrays demonstrated spreading and increased growth for up to 40 days, and their orientation followed that of the aligned fibers. The possibility to combine the collagen cellular arrays with poly(ethylene glycol) diacrylate (PEG-DA) hydrogel, to create integrated biocomposites, was also demonstrated. This study showed that coral collagen fibers in combination with a hydrogel can support biological tissue-like growth, with predefined orientation over a long period of time in culture. As such, it is an attractive scaffold for the construction of various engineered tissues to match their native oriented morphology.

中文翻译：

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用于工程对齐组织的珊瑚衍生胶原纤维

越来越需要生物材料支架来支持软组织替代品的工程设计，其结构和机械性能与天然组织相似。这项工作介绍了一种新的生物材料系统，该系统基于从肉癣菌中提取的厘米长的胶原纤维。软珊瑚，缠绕在框架上以创建对齐的纤维阵列。胶原阵列显示出类似于富含胶原蛋白的组织的超弹性和粘弹性机械特性。细胞毒性试验表明，胶原阵列对成纤维细胞无毒。此外，接种在胶原阵列上的成纤维细胞表现出长达 40 天的扩散和生长增加，并且它们的方向遵循对齐的纤维的方向。还证明了将胶原细胞阵列与聚（乙二醇）二丙烯酸酯（PEG-DA）水凝胶结合以创建集成生物复合材料的可能性。这项研究表明，珊瑚胶原纤维与水凝胶相结合，可以支持生物组织样生长，并在培养的很长一段时间内具有预定义的方向。像这样，