Multidrug-resistant extended-spectrum beta lactamase (ESBL)-producing Escherichia coli strains are emerging globally in both humans and animals. Antimicrobial susceptibility testing and ESBL screening were performed on pure cultures of 216 E. coli isolates from human and animal fecal samples as well as beef. Polymerase chain reaction was performed for the detection of resistance genes. Representative isolates of ESBL-producing E. coli were randomly selected for multilocus sequence typing and pulsed field gel electrophoresis (PFGE). Sixty of the isolates were identified as ESBL producers, and seven resistance genes were amplified in them: TEM (61.7%), blaCTX-M-15 (51.7%), AAC-6-LB (43.3%), blaCTX-M-1 (38.3%), blaCTX-M-9 (33.3%), blaCTX-M-2 (21.7%), and SHV (11.7%); they were classified into four phylogroups: A (25%), B1 (45%), B2 (20%), and D (10%). Thirty of these isolates were clustered into 10 sequence types with ST131 being mostly prevalent. Six PFGE types were discovered, each of which was shared by isolates from different subjects and had the same phylogroups and resistance gene profiles. There was a dissemination of PFGE types across various groups among humans, animals, and beef. This underlines the fact that the spread of ESBL E. coli could be from humans to animals, from animals to humans, as well as across animal species.

中文翻译：

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来自尼日利亚西南部食品生产动物、牛肉和人类的产超广谱 β 内酰胺酶的大肠杆菌的系统发育特征和多位点序列分型。

产生多重耐药性的超广谱 β 内酰胺酶 (ESBL)大肠杆菌菌株正在全球范围内在人类和动物中出现。对来自人类和动物粪便样本以及牛肉的 216株大肠杆菌的纯培养物进行了抗微生物药敏试验和 ESBL 筛选。聚合酶链反应用于检测抗性基因。随机选择产 ESBL大肠杆菌的代表性分离株进行多位点序列分型和脉冲场凝胶电泳 (PFGE)。其中 60 个分离株被鉴定为 ESBL 生产者，并在其中扩增了 7 个抗性基因：TEM (61.7%)、blaCTX-M-15 (51.7%)、AAC-6-LB(43.3%)、blaCTX-M-1 (38.3%)、blaCTX-M-9 (33.3%)、blaCTX-M-2 (21.7%) 和SHV (11.7%)；它们被分为四个系统群：A (25%)、B1 (45%)、B2 (20%) 和 D (10%)。这些分离株中有 30 株被分为 10 种序列类型，其中 ST131 最为普遍。发现了六种 PFGE 类型，每种类型都由来自不同受试者的分离株共享，并且具有相同的系统群和抗性基因谱。PFGE 类型在人类、动物和牛肉的不同群体中传播。这强调了 ESBL大肠杆菌的传播可能从人类到动物、从动物到人类以及跨动物物种的事实。