The aim of this study was to evaluate the antibacterial properties of methylsulfonylmethane (MSM) on vancomycin-resistant Enterococcus faecium (VRE). Bacterial proliferation was measured spectrophotometrically during growth in brain heart infusion broth with 0%, 3%, 5%, 7%, 10%, 12%, and 16% MSM. To assess the mechanism of inhibition, VRE was grown overnight with 0–16% MSM and enumerated on unmedicated and medicated (3–16% MSM) brain heart infusion agar (BHIA). Viability studies were performed to evaluate the impact of 10–16% MSM on VRE over 7 days. Absorbance data indicated a dose-dependent inhibition from 0% to 7% MSM and no increase in optical density in 10–16% MSM. VRE enumerated on unmedicated BHIA from overnight cultures with 10–16% MSM partially recovered. No growth was observed when BHIA contained 10–16% MSM. There was little effect on VRE growth in 10% MSM over 7 days. VRE displayed a population rebound on day 6 when exposed to 12% MSM, and elimination by day 6 in 16% MSM. Regrowth after MSM removal may be indicative of a bacteriostatic mechanism of inhibition. Cell elimination in 16% MSM suggests inhibition of an essential metabolic function from which the bacterium could not recover.

中文翻译：

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甲基磺酰甲烷在体外表现出对万古霉素抗性屎肠球菌的抑菌抑制作用。

本研究的目的是评估甲基磺酰甲烷 (MSM) 对耐万古霉素屎肠球菌的抗菌特性。(VRE)。在含 0%、3%、5%、7%、10%、12% 和 16% MSM 的脑心浸液肉汤中生长期间，通过分光光度法测量细菌增殖。为了评估抑制机制，VRE 与 0-16% MSM 一起生长过夜，并在未加药和加药（3-16% MSM）脑心浸液琼脂（BHIA）上计数。进行活力研究以评估 10-16% MSM 对 VRE 7 天的影响。吸光度数据表明从 0% 到 7% MSM 的剂量依赖性抑制，并且在 10-16% MSM 中光密度没有增加。从过夜培养物中对未加药的 BHIA 计数的 VRE，其中 10-16% 的 MSM 部分恢复。当 BHIA 含有 10-16% 的 MSM 时，没有观察到增长。在 7 天内，10% MSM 对 VRE 的增长几乎没有影响。当暴露于 12% MSM 时，VRE 在第 6 天显示人口反弹，并在 16% MSM 中第 6 天消除。MSM 去除后的再生可能表明抑制的抑菌机制。16% MSM 中的细胞消除表明抑制了细菌无法从中恢复的基本代谢功能。