ABSTRACT

Ultrasound has the ability to be concentrated into a limited area of a tumor to activate a sonosensitizer. To develop the effectiveness of sonodynamic therapy of cancer utilizing sonosensitizers, we improved an innovative titanium dioxide (TiO₂) nanoparticles functionalized porphyrin and discussed its features and sonotoxicity on tumor cells. Briefly, TiO₂nanoparticle has been created by means of titanium tetrahydroperoxide precursor. The surface of TiO₂ was modified with polyvinyl alcohol (PVA) polymer and subsequently with porphyrin. TEM, DLS, FTIR, and XRD were employed to examine the characteristics of samples. Cytotoxicity of the samples was determined using MTT assay test in vitro.The therapeutic effect of sonodynamic therapy (SDT) via TiO₂-based nanoparticles on breast cancer were evaluatedin vitro using MDA-MB-231 cell line.The results of the MTT test indicate that TiO₂ and the porphyrin-containing samples are non-toxic but in combination with ultrasound radiation are more effective in killing breast cancer cells.

摘要

超声波能够集中到肿瘤的有限区域以激活声敏剂。为了开发利用声敏剂治疗癌症的声动力疗法的有效性，我们改进了一种创新的二氧化钛 (TiO ₂ ) 纳米颗粒功能化卟啉，并讨论了其特性和对肿瘤细胞的声毒性。简而言之，TiO ₂纳米颗粒是通过四氢过氧化钛前体产生的。TiO ₂ 的表面用聚乙烯醇（PVA）聚合物改性，随后用卟啉改性。采用 TEM、DLS、FTIR 和 XRD 来检查样品的特性。样品的细胞毒性通过体外MTT试验测定。通过TiO ₂的声动力疗法（SDT）的治疗效果使用MDA-MB-231细胞系体外评估基于纳米粒子对乳腺癌的作用。MTT测试结果表明TiO ₂ 和含卟啉样品无毒，但与超声辐射联合使用更有效地杀死乳腺癌癌细胞。