Introduction/Objective: Differently from other digestive malignancies, gastric cancer (GC) pathobiology is still little known and understood. Recently, cytopathology and molecular biology on gastric juice/gastric lavage (GJ/GL) of GC patients have provided novel and interesting results in terms of screening, diagnosis, prognosis, and therapy. However, entertaining cytologic examination and molecular test as a unified solo-run test is previously unreported. Our aim was to assess the new parameter “GL Ca 72.4” for GC patients. Methods: Between April 2012 and July 2013, GJ/GL obtained from 37 surgical GC patients were tested for the presence/absence (GL1/GL0) of exfoliated malignant cells along with the intragastric concentration of Ca 72.4 (normal value #x3c;6.49 ng/mL: Ca 72.4n; elevated level ≥6.49 ng/mL: Ca 72.4+). Results: At a median follow-up of 79.3 months, all the GC alive patients were “GL0 Ca 72.4n.” The “GL1 Ca 72.4+” parameter, in comparison with GL0 Ca 72.4n, strongly correlated with deeper tumor invasion (p = 0.027), severe nodal metastasis (p = 0.012), worst metastatic node ratio (p = 0.041), higher number of metastatic lymph nodes (30 vs. 20 nodes, p = 0.014), angiolymphatic invasion (p = 0.044), advanced stage (p = 0.034), and adjuvant therapy (p = 0.044). The Kaplan-Meier model showed that GL1 Ca 72.4+ subjects had shorter overall survival (OS) than GL0 Ca 72.4n cases (9.7 vs. 43.2 months, respectively, p = 0.042). At univariate analysis, the GL1 Ca 72.4+ parameter resulted a significant prognostic factor for OS (p = 0.023). Conclusions: The combined cyto-molecular parameter “GL1 Ca 72.4+” appears to be a strong indicator of aggressive tumor behavior and a significant prognostic factor of poor survival for GC patients.
Acta Cytologica

中文翻译：

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胃腺癌中胃恶性剥脱和Ca 72.4浓度的组合分析：“ GL1 Ca 72.4”参数。

简介/目的：与其他消化系统恶性肿瘤不同，胃癌（GC）的病理生物学仍知之甚少。近来，关于GC患者胃液/灌胃（GJ / GL）的细胞病理学和分子生物学在筛查，诊断，预后和治疗方面提供了新颖而有趣的结果。但是，以前尚未报道过将细胞学检查和分子测试作为统一的单独运行测试的方法。我们的目的是评估GC患者的新参数“ GL Ca 72.4”。方法：在2012年4月至2013年7月之间，对从37名外科手术GC患者中获得的GJ / GL进行了检测，以观察是否存在脱落的恶性细胞（GL1 / GL0）以及胃内Ca 72.4的浓度（正常值＃x3c； 6.49 ng / mL ：Ca 72.4n；升高的浓度≥6.49ng / mL：Ca 72.4+）。结果：在中位随访79.3个月时，所有GC存活患者均为“ GL0 Ca 72.4n”。与GL0 Ca 72.4n相比，“ GL1 Ca 72.4+”参数与更深的肿瘤浸润（p = 0.027），严重的淋巴结转移（p = 0.012），最差的转移淋巴结比率（p = 0.041），更高的数值密切相关。转移淋巴结的数目（30个与20个结点，p = 0.014），淋巴管浸润（p= 0.044），晚期（p = 0.034）和辅助治疗（p = 0.044）。Kaplan-Meier模型显示，GL1 Ca 72.4+受试者的总生存期（OS）比GL0 Ca 72.4n病例要短（分别为9.7和43.2个月，p = 0.042）。在单变量分析中，GL1 Ca 72.4+参数导致OS的重要预后因素（p = 0.023）。结论：组合的细胞分子参数“ GL1 Ca 72.4+”似乎是侵袭性肿瘤行为的有力指标，也是GC患者生存不良的重要预后因素。
细胞学学报