The aim of this study was to examine whether a morphological approach is efficient for selecting high‐quality porcine embryos produced by in vitro fertilization (IVF) under high polyspermy conditions. Frozen‐thawed Meishan epididymal spermatozoa showing moderate and high polyspermy were subjected to IVF (1 × 10⁵ sperms/ml). Under conditions of moderate polyspermy, 4‐cell embryos selected at 48 hr after IVF (single selection) and 8‐cell embryos selected at 79 hr after IVF from the collected 4‐cell embryos (double selection) showed high developmental competence. Likewise, 4‐ and 8‐cell embryos produced by IVF under high polyspermy conditions also showed high competence for development to blastocysts. However, blastocysts derived from high polyspermy conditions had significantly fewer cells than those produced under moderate polyspermy conditions. Furthermore, the frequency of nuclear and chromosomal abnormalities in 4‐ and 8‐cell embryos produced under conditions of high polyspermy was significantly (p  < .05) higher in comparison to moderate polyspermy conditions. These findings suggest that although high polyspermy affects the frequency of nuclear and chromosomal anomalies in porcine IVF embryos, subsequent selection based on morphological features of 4‐ and 8‐cell embryos even under high polyspermy conditions, could be an alternative option for selecting porcine IVF embryos with high development ability.

中文翻译：

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基于体外受精产生的猪胚胎形态特征的选择：早期卵裂的时间和多精子的影响。

本研究的目的是检验形态学方法是否能有效地选择在高多精条件下通过体外受精 (IVF) 产生的高质量猪胚胎。冷冻解冻的眉山附睾精子表现出中度和高度多精子进行 IVF (1 × 10 ⁵ 精子/毫升）。在中度多精条件下，IVF 后 48 小时选择的 4 细胞胚胎（单选）和 IVF 后 79 小时从收集的 4 细胞胚胎中选择的 8 细胞胚胎（双选）显示出较高的发育能力。同样，在高多精子条件下通过 IVF 产生的 4 细胞和 8 细胞胚胎也显示出高度的胚泡发育能力。然而，与在中等多精子条件下产生的囊胚相比，高多精子条件下产生的囊胚的细胞数量要少得多。此外，在高多精条件下产生的 4 细胞和 8 细胞胚胎的核和染色体异常频率显着（p < .05) 与中度多精条件相比更高。这些发现表明，虽然高多精子影响猪 IVF 胚胎的核和染色体异常的频率，但即使在高多精子条件下，基于 4 细胞和 8 细胞胚胎形态学特征的后续选择也可能是选择猪 IVF 胚胎的替代选择具有较高的开发能力。