Resin‐based reconstructive/restorative materials with antibacterial effects are potentially useful for preventing dental and oral diseases. To this end, the immobilization of an antibacterial component on the surface of a resin by incorporating polymerizable bactericide such as a quaternary ammonium compound‐monomer 12‐methacryloyloxydodecylpyridinium bromide (MDPB) is an effective technique. However, the effectiveness of immobilized bactericide is reduced by salivary protein coverage. We address this issue by utilizing 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer, which exhibits protein repellency, with MDPB to fabricate a novel copolymer, which served as a surface coating on a methacrylate‐based resin. This coating provided a more hydrophilic surface than that provided by MDPB coating and reduced the adsorption of bovine serum albumin and salivary protein. To evaluate bacterial growth on the contact surface, Streptococcus mutans suspension was placed on the coated specimen. After 24‐h incubation, MDPB/MPC copolymer exhibited killing effects against S. mutans. Moreover, confocal laser scanning microscopy and scanning electron microscopy were used to evaluate biofilm formation after 48‐h incubation in S. mutans suspension, which revealed sparse biofilm and dead bacteria in biofilm on the surface coated with MDPB/MPC. Overall, the proposed surface coating on dental resins exhibited protein‐repellent ability and inhibitory effects against bacteria and oral biofilms.

中文翻译：

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开发具有抗菌活性和蛋白质排斥双重功能的由 MDPB 和 MPC 组成的新型表面涂层。

具有抗菌作用的树脂基重建/修复材料可能有助于预防牙科和口腔疾病。为此，通过掺入可聚合杀菌剂，如季铵化合物-单体 12-甲基丙烯酰氧基十二烷基溴化吡啶鎓（MDPB），将抗菌成分固定在树脂表面是一种有效的技术。然而，固定化杀菌剂的有效性会因唾液蛋白覆盖而降低。我们通过使用具有蛋白质排斥性的 2-甲基丙烯酰氧乙基磷酰胆碱 (MPC) 聚合物和 MDPB 来制造一种新型共聚物来解决这个问题，该共聚物用作甲基丙烯酸酯基树脂的表面涂层。这种涂层提供了比 MDPB 涂层更亲水的表面，并减少了牛血清白蛋白和唾液蛋白的吸附。为了评估接触面上的细菌生长，将变形链球菌悬浮液置于包被的标本上。孵育 24 小时后，MDPB/MPC 共聚物表现出对变形链球菌的杀灭作用。此外，共聚焦激光扫描显微镜和扫描电子显微镜被用来评估在变形链球菌悬浮液中孵育 48 小时后生物膜的形成，这表明在涂有 MDPB/MPC 的表面上的生物膜中存在稀疏的生物膜和死细菌。总体而言，所提出的牙科树脂表面涂层表现出蛋白质排斥能力和对细菌和口腔生物膜的抑制作用。