Vitreous humor (VH) is an important alternative matrix used in post-mortem forensic toxicology. VH has particular advantages over other biological matrices due to its good resistance to autosynthesis of endogenous compounds in post-mortem processes. However, the analysis of VH samples is mainly based on classical extraction techniques at preparation stage and chromatographic methods used to determine psychoactive substances. There is a lack of new, quicker and more effective drug extraction techniques, which could also correspond to the current trends in Green Chemistry.

The aim of this study is to summarize and critically review the data submitted for post-mortem analysis of VH for toxicological and forensic purposes. It deals with some important issues: sampling, storage, preparation, and methods for identifying different important types of psychoactive drugs. In addition, the review provides insight into the pharmacokinetics of xenobiotics and highlights the main trends and achievements in correlation studies with classical matrices.

玻璃体液（VH）是验尸法医毒理学中使用的重要替代基质。VH与其他生物基质相比具有特殊的优势，因为它对尸体解剖过程中内源性化合物的自动合成具有良好的抵抗力。但是，VH样品的分析主要基于制备阶段的经典提取技术和用于确定精神活性物质的色谱方法。缺少新的，更快的和更有效的药物提取技术，这也可能与绿色化学的当前趋势相对应。

这项研究的目的是总结并严格审查为毒理学和法医目的对VH进行死后分析而提交的数据。它涉及一些重要问题：采样，存储，制备以及用于识别不同重要类型的精神活性药物的方法。此外，该综述提供了对异生物素药代动力学的见解，并突出了与经典基质相关研究的主要趋势和成就。