Abstract Cardiovascular disease remains the leading cause of death. Damaged heart muscle is the etiology of heart failure. Heart failure is the most frequent cause of hospital and emergency room admissions. As a differentiated organ, current therapeutics and techniques can not repair or replace the damaged myocardial tissue. Myocardial tissue engineering is one of the promising treatment modalities for repairing damaged heart tissue in patients with heart failure. In this work, random Polylactic acid (PLA), Polylactic acid/Polyethylene glycol (PLA/PEG) and random and aligned Polylactic acid/Polyethylene glycol/Collagen (PLA/PEG/COL) nanofiber patches were successfully produced by the electrospinning technique. In vitro cytotoxic test (MTT), morphological (SEM), molecular interactions between the components (FT-IR), thermal analysis (DSC), tensile strength and physical analysis were carried out after production. The resulting nanofiber patches exhibited beadless and smooth structures. When the fiber diameters were examined, it was observed that the collagen doped random nanofiber patches had the lowest fiber diameter value (755 nm). Mechanical characterization results showed that aligned nanofiber patches had maximum tensile strength (5.90 MPa) values compared to PLA, PLA/PEG, and PLA/PEG/COL (random). In vitro degradation test reported that aligned patch had the highest degradation ratio. The produced patches displayed good alignment with tissue on cardiomyocyte cell morphology studies. In conclusion, newly produced patches have noticeable potential as a tissue-like cardiac patch for regeneration efforts after myocardial infarction.

中文翻译：

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用于心肌组织工程的弹性心脏组织样贴片的生产和表征

摘要 心血管疾病仍然是导致死亡的主要原因。受损的心肌是心力衰竭的病因。心力衰竭是住院和急诊室入院的最常见原因。作为分化器官，目前的治疗方法和技术无法修复或替代受损的心肌组织。心肌组织工程是修复心力衰竭患者受损心脏组织的有前途的治疗方式之一。在这项工作中，通过静电纺丝技术成功地生产了无规聚乳酸（PLA）、聚乳酸/聚乙二醇（PLA/PEG）和无规排列的聚乳酸/聚乙二醇/胶原蛋白（PLA/PEG/COL）纳米纤维贴片。体外细胞毒性试验（MTT）、形态学（SEM）、组分间分子相互作用（FT-IR）、热分析（DSC）、生产后进行抗拉强度和物理分析。由此产生的纳米纤维贴片表现出无珠和光滑的结构。当检查纤维直径时，观察到胶原掺杂的随机纳米纤维贴片具有最低的纤维直径值（755 nm）。机械表征结果表明，与 PLA、PLA/PEG 和 PLA/PEG/COL（随机）相比，对齐的纳米纤维贴片具有最大的拉伸强度 (5.90 MPa) 值。体外降解试验表明，对齐的贴片具有最高的降解率。在心肌细胞形态学研究中，产生的贴片显示出与组织的良好对齐。总之，新生产的贴片作为心肌梗塞后再生努力的组织样心脏贴片具有显着的潜力。