Templating has been demonstrated to be an efficient method of nanocapsule preparation. However, there have been no reports of using protein-only nanocapsules as an antigen delivery system. Such a system would enable the delivery of antigen without additional polymers. This study focused on defining the structural and cellular characteristics of nanocapsules consisting of antigen (ovalbumin) alone, synthesized by the templating method using highly monodispersed solid core mesoporous shell (SC/MS) and mesoporous (MS) silica nanoparticles of 410 nm and 41 nm in diameter, respectively. The synthesized ovalbumin nanocapsules were homogeneous in structure, and cellular uptake was observed in DC2.4 murine immature dendritic cells with minimal cytotoxicity. The nanocapsules were localized intracellularly and induced antigen presentation by the cross-presentation pathway. The templating system, using SC/MS and MS silica nanoparticles, was demonstrated to be an effective nanocapsule synthesis method for a new antigen delivery system.

已证明模板是纳米胶囊制备的有效方法。然而，没有报道使用仅蛋白质的纳米胶囊作为抗原递送系统。这样的系统将能够递送抗原而无需另外的聚合物。这项研究的重点是确定仅由抗原（卵清蛋白）组成的纳米胶囊的结构和细胞特征，该纳米胶囊通过模板方法使用高度单分散的固体中孔壳（SC / MS）和中孔（MS）二氧化硅纳米颗粒（410 nm和41 nm）合成直径分别。合成的卵清蛋白纳米胶囊结构均匀，在DC2.4鼠未成熟树突状细胞中观察到细胞摄取，细胞毒性最小。纳米胶囊位于细胞内，并通过交叉呈递途径诱导抗原呈递。使用SC / MS和MS二氧化硅纳米颗粒的模板系统被证明是一种用于新型抗原递送系统的有效纳米胶囊合成方法。