In this study, the influence of moisture and acids on the content of fatty acid methyl esters and triacetin in the interesterification reaction by using 1M potassium tert-butoxide in tetrahydrofuran as a homogeneous catalyst was investigated. During the interesterification reaction, the presence of moisture causes a larger negative effect than free fatty acids or acetic acid. The highest content of fatty acid methyl esters (77.6 wt%) and triacetin (17.4 wt%) was achieved at methyl acetate to oil molar ratio (MAOMR) 60 and catalyst to oil molar ratio (COMR) 0.05 by using raw materials with moisture and acids content no larger than 0.005% and 0.08%, respectively.

During the interesterification reaction, the excess of the catalyst caused the formation of two-layer and its proceeds only by using high-quality raw materials. This fact has never been mentioned in the literature. The upper layer is biodiesel, the lower layer consists of glycerol and glycerol acetates (mono-, di-, and triacetin). The COMR has a more significant effect on the formation of the biodiesel and glycerol acetates layers than MAOMR. The highest yield of glycerol acetates layer (19.4%) has been formed by using MAOMR 60 and COMR 0.3.

本研究以四氢呋喃中的1M叔丁醇钾为均相催化剂，研究了水分和酸对酯交换反应中脂肪酸甲酯和甘油三乙酸酯含量的影响。在酯交换反应期间，水分的存在比游离脂肪酸或乙酸引起更大的负面影响。通过使用具有水分和水分的原料，在乙酸甲酯与油的摩尔比（MAOMR）60和催化剂与油的摩尔比（COMR）0.05的情况下，脂肪酸甲酯（77.6 wt％）和三醋精（17.4 wt％）的含量最高。酸含量分别不大于0.005％和0.08％。

在酯交换反应过程中，过量的催化剂导致两层的形成，并且仅通过使用高质量的原材料就可以进行两步反应。这个事实在文献中从未提及。上层是生物柴油，下层由甘油和乙酸甘油酯（单，双和三醋精）组成。与MAOMR相比，COMR对生物柴油和乙酸甘油酯层的形成具有更大的影响。通过使用MAOMR 60和COMR 0.3，可以形成最高产率的乙酸甘油酯层（19.4％）。