Sequence analysis of the genomic DNA isolated from four biotypes of the soybean aphid, Aphis glycines (AG), revealed that in addition to the commonly observed retrovirus-related retrotransposons, viral sequences derived from multiple RNA and DNA viruses have integrated into the genome. Notably, sequences of more than 60 nudiviral genes were identified from de novo assembled DNA contigs, and mapped to assembled genomic scaffolds of AG, indicating that an ancient nudivirus, named Aphis glycines endogenous nudivirus (AgENV), had integrated into the AG genome. Furthermore, sequences derived from a similar endogenous nudivirus, Melanaphis sacchari endogenous nudivirus (MsENV), were identified from the genomic scaffolds of the sugarcane aphid, Melanaphis sacchari. Analysis of transcriptome and small RNA sequence data derived from AG did not provide evidence for transcription of the integrated AgENV genes. Hence, the genes of AgENV may be present as pseudogenes. Phylogenetic analysis based on nudivirus core genes indicated that these aphid ENVs belong to the genus Alphanudivirus.

对从四种生物型大豆蚜虫（AG）中分离的基因组 DNA 进行序列分析表明，除了常见的逆转录病毒相关逆转录转座子外，源自多种 RNA 和 DNA 病毒的病毒序列也已整合到基因组中。值得注意的是，从头组装的 DNA 重叠群中鉴定出了 60 多个裸病毒基因的序列，并将其映射到 AG 的组装基因组支架上，表明一种古老的裸病毒，称为蚜虫内源性裸病毒（AgENV），已经整合到 AG 基因组中。此外，从甘蔗蚜虫Melanaphis sacchari的基因组支架中鉴定出源自类似内源性裸病毒（ Melanaphis sacchari 内源性裸病毒（MsENV））的序列。对来自 AG 的转录组和小 RNA 序列数据的分析没有提供整合的 AgENV 基因转录的证据。因此，AgENV 的基因可能以假基因的形式存在。基于裸病毒核心基因的系统发育分析表明，这些蚜虫ENV属于Alphanudivirus属。