1-Oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) is a critical structured lipid in human milk fat and it plays an important role in infants' health. The development of an efficient method for such compound preparation is necessary. In this study, one- and two-step methods for OPL production by enzymatic acidolysis of fractionated palm stearin with mixed acids were compared in solvent-free and solvent systems. The results demonstrated that in the solvent system, the OPL contents by the one- and two-step methods were 45.8% and 56.7%, respectively. In the solvent-free system, the one- and two-step methods resulted in the formation of 43.7% and 54.8% OPL, respectively. Subsequently, acidolysis conditions of the two-step method were optimized. Under the optimum conditions, the content of OPL was 57.7%, and 83.2% of palmitic acid was at the sn-2 position. The prepared OPL has potential to be applied in infant formulas.

1-油酰基-2-棕榈酰基-3-亚油酰基甘油（OPL）是人乳脂肪中的关键结构脂质，对婴儿的健康起着重要作用。需要开发用于这种化合物制备的有效方法。在这项研究中，在无溶剂和溶剂体系中比较了用混合酸进行分级分离的棕榈硬脂精的酶法酸解生产OPL的一步法和两步法。结果表明，在溶剂体系中，一步法和两步法的OPL含量分别为45.8％和56.7％。在无溶剂系统中，一步法和两步法分别形成了43.7％和54.8％的OPL。随后，优化了两步法的酸解条件。在最佳条件下，OPL的含量为57.7％，为83。2％的棕榈酸在sn-2位置。制备的OPL有潜力应用于婴儿配方食品中。