CLEC12B is a C-type lectin-like receptor expressed on myeloid cells. In this study, we have characterized the porcine homologue of CLEC12B (poCLEC12B). To this end, we have generated constructs encoding a c-myc tagged version of the whole receptor, or its ectodomain fused to the Fc portion of human IgG1, from a cDNA clone obtained from an alveolar macrophage library, and raised monoclonal antibodies (mAb) against this molecule. Using these mAbs, poCLEC12B was found to be expressed on alveolar macrophages and, at lower levels, on blood conventional type 1 dendritic cells (cDC1) and plasmacytoid DCs. No binding was detected on monocytes, monocyte-derived macrophages or monocyte-derived DCs. Engagement of CLEC12B on alveolar macrophages with mAbs had no apparent effect on cytokine production (TNF-α, IL-8) induced by LPS. These results provide the basis for future investigations aimed to assess the role of poCLEC12B in different microbial infections and to evaluate its potential in vaccination strategies targeting DCs.

CLEC12B 是一种在骨髓细胞上表达的 C 型凝集素样受体。在这项研究中，我们对 CLEC12B (poCLEC12B) 的猪同系物进行了表征。为此，我们从肺泡巨噬细胞文库中获得的 cDNA 克隆中生成了编码整个受体的 c-myc 标记版本或其胞外域与人 IgG1 Fc 部分融合的构建体，并产生了单克隆抗体 (mAb)反对这个分子。使用这些 mAb，发现 poCLEC12B 在肺泡巨噬细胞上表达，并以较低水平在血液常规 1 型树突细胞 (cDC1) 和浆细胞样 DC 上表达。在单核细胞、单核细胞衍生的巨噬细胞或单核细胞衍生的 DC 上未检测到结合。CLEC12B 在肺泡巨噬细胞上与 mAb 的结合对 LPS 诱导的细胞因子产生（TNF-α、IL-8）没有明显影响。