Abstract Self-assembly of nanoparticles has become a valuable tool for creation of various nano-textured materials for biomedicine, electronics, optics, photonics and biotechnology. In this paper, polystyrene sulfonate (PSS) modified tubular and platy nanoclays were used for fabrication of concentric ring patterns on solid surfaces based on "coffee-ring" effect. The technique of nanoclay self-assembly into concentric structures described in this paper does not require special equipment and the self-assembly process is rather fast (1-2 h). Two different molds (a metal sphere and a glass pyramid) fixed in close proximity above a cover glass were used for creating confined geometries necessary to control the process of clay suspension evaporation. The resulting concentric patterns were applied for orienting the growth of human skin fibroblasts and capturing cancer and protozoan cells. The concentric structures formed using HNT-PSS suspension and the pyramid were more effective in directing the growth of fibroblasts, while HNT-PSS and KNP-PSS coatings formed by the sphere fitted better for cell capturing.

中文翻译：

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用于细胞图案化和捕获的小管和板状纳米粘土同心微环的自组装

摘要 纳米粒子的自组装已成为创造用于生物医学、电子学、光学、光子学和生物技术的各种纳米纹理材料的宝贵工具。在本文中，聚苯乙烯磺酸盐 (PSS) 改性管状和板状纳米粘土用于基于“咖啡环”效应在固体表面上制造同心环图案。本文描述的纳米粘土自组装成同心结构的技术不需要特殊设备，自组装过程相当快（1-2 小时）。两个不同的模具（一个金属球和一个玻璃金字塔）紧密地固定在盖玻片上方，用于创建控制粘土悬浮液蒸发过程所需的受限几何形状。由此产生的同心圆图案用于定向人类皮肤成纤维细胞的生长并捕获癌症和原生动物细胞。使用 HNT-PSS 悬浮液和金字塔形成的同心结构在指导成纤维细胞生长方面更有效，而由球体形成的 HNT-PSS 和 KNP-PSS 涂层更适合细胞捕获。