Masson pine ( Pinus massoniana Lamb.) is an economically important conifer tree that can be widely used for timber, pulp, and resin production. However, the phosphate (Pi) deficiency in tropical and subtropical forest soils poses severe challenges for the productivity of masson pine. WRKY transcription factors (TFs) have been proven to play important roles in plant responses to biotic and abiotic stresses, including low Pi stress; however, little is known about their roles in masson pine. To understand the roles of P. massoniana WRKY (PmWRKY) in low Pi stress, 25 putative WRKY TFs with complete WRKY domain from transcriptome sequencing data were identified. Based on their conserved domains and zinc-finger motif, the P. massoniana WRKY were divided into three groups. Structural feature analysis shows that PmWRKY proteins contain WRKYGQK/GKK/GRK domains and a C 2 H 2 /C 2 HC-type zinc-finger structure. To putatively identify the function of PmWRKY members involved in low Pi stress, transcriptional profiles of 17 PmWRKYs in masson pine under different Pi stresses were systematically established using real-time quantitative RT-PCR analysis. Analysis demonstrates that the candidate PmWRKYs were involved in responses to Pi starvation—for example, PmWRKY11 , 12 , and 13 were upregulated both in P 1 (Phosphorus content of 0.01 mM) and P 2 (Phosphorus content of 0.06 mM) stresses. The available evidence conclusively sheds light on protein structures, evolutionary relationships, and expression patterns of WRKYs in response to low Pi stress of masson pine, which facilitates further functional identification and molecular breeding for the enhancement of low-phosphorous tolerance in this species.

中文翻译：

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马尾松 WRKY 转录因子在低磷胁迫下的全转录组鉴定和表达谱

马尾松（Pinus massoniana Lamb.）是一种经济上重要的针叶树，可广泛用于木材、纸浆和树脂生产。然而，热带和亚热带森林土壤中磷酸盐（Pi）的缺乏对马尾松的生产力构成了严峻挑战。WRKY 转录因子 (TFs) 已被证明在植物对生物和非生物胁迫（包括低磷胁迫）的反应中发挥重要作用；然而，人们对它们在马尾松中的作用知之甚少。为了了解马尾松 WRKY (PmWRKY) 在低 Pi 胁迫中的作用，从转录组测序数据中鉴定了 25 个具有完整 WRKY 结构域的推定 WRKY TF。根据它们的保守结构域和锌指基序，马尾松 WRKY 被分为三组。结构特征分析表明PmWRKY蛋白含有WRKYGQK/GKK/GRK结构域和C 2 H 2 /C 2 HC型锌指结构。为了推测 PmWRKY 成员参与低磷胁迫的功能，使用实时定量 RT-PCR 分析系统地建立了马尾松在不同磷胁迫下 17 个 PmWRKY 的转录谱。分析表明，候选 PmWRKY 参与了对磷饥饿的反应——例如，PmWRKY11、12 和 13 在 P 1（磷含量为 0.01 mM）和 P 2（磷含量为 0.06 mM）胁迫中均上调。现有证据最终阐明了 WRKYs 响应马尾松低磷胁迫的蛋白质结构、进化关系和表达模式，