Abstract—One of the components of the cytoskeleton is microtubules (MT), a complex intracellular system, damage to which may cause disturbances that lead to the development of pathological processes in the cell. This gives rise to the study of tubulin, the main MT protein, in schizophrenia. To assess the relative amount of tubulin, its specific property to bind colchicine at an equimolar ratio was used. The aim of the study was to determine the level of colchicine binding (the colchicine-binding activity of tubulin) in various brain structures under normal conditions and in schizophrenia. The study was performed with samples of the structures of the postmortem brain of patients with schizophrenia (n = 7) and individuals in the control group (n = 15). The colchicine-binding activity of tubulin was determined using the Scherline method in tubulin-rich protein extracts (a single polymerization-depolymerization). In the prefrontal (area 10), limbic (area 23/24), and temporal (area 21) cerebral cortex of patients with schizophrenia, a significant decrease in the colchicine-binding activity of tubulin was observed in comparison with the control. In the thalamus, colchicine-binding activity in schizophrenia did not change relative to the control, but it was lower than in the areas of the cortex. In schizophrenia, we observed a decrease in the colchicine-binding activity of tubulin in the prefrontal (area 10), limbic (area 23/24), and temporal (area 21) cerebral cortex but not in the thalamus (subcortical structure of the brain).

中文翻译：

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正常情况下和精神分裂症中秋水仙碱与微管蛋白的结合

摘要—细胞骨架的组成部分之一是微管（MT），这是一个复杂的细胞内系统，对其破坏可能会引起干扰，从而导致细胞病理过程的发展。这引起了精神分裂症中主要的MT蛋白微管蛋白的研究。为了评估微管蛋白的相对量，使用了其以等摩尔比结合秋水仙碱的特异性。该研究的目的是确定正常情况下和精神分裂症中各种脑结构中秋水仙碱的结合水平（微管蛋白的秋水仙碱结合活性）。这项研究是对精神分裂症患者（n = 7）和对照组（n= 15）。微管蛋白的秋水仙碱结合活性是通过Scherline方法在富含微管蛋白的蛋白质提取物中进行的（一次聚合-解聚）。在患有精神分裂症的患者的前额叶（区域10），边缘（区域23/24）和颞部（区域21）大脑皮层中，与对照相比，观察到微管蛋白的秋水仙碱结合活性显着降低。在丘脑中，精神分裂症中的秋水仙碱结合活性相对于对照没有变化，但是低于皮质区域。在精神分裂症中，我们观察到前额叶（区域10），边缘（区域23/24）和颞部（区域21）大脑皮层中微管蛋白的秋水仙碱结合活性降低，而丘脑（大脑皮层下结构）则未降低）。