Abstract

Our aim was to determine the levels of arginine decarboxylase (ADC), ornithine decarboxylase (ODC) and agmatine, ornithine, nitric oxide (NO) and arginase activity in the acute and subacute phases of acute ischemic stroke (AIS), by revealing the possible role of these levels in neuroinflammation and neurogenesis that are postulated to begin after AIS. 35 patients diagnosed with AIS and 35 controls were included in the study. ADC, ODC, agmatinase and NO levels were determined by ELISA kits. Arginase activity and ornithine levels were measured spectrophotometrically. There was no statistically significant difference between the levels of agmatinase, ornithine and arginase activity on days 1 and 7 in the AIS group and the levels measured in the control group (p > 0.05). High ADC and low ODC and NO values were found in the patients compared to the controls in both acute and subacute periods (p < 0.05). It was shown that polyamine synthesis decreased in both acute and subacute periods after AIS; and this was due to the decrease in ODC levels, the rate-regulating step of the major pathway. This reduction may be associated with both the inadequate stimulation of neurogenesis and the immune system with reduced NO synthesis.

Keywords:

polyamine synthesis enzymes, ornithine, ornitine decarboxylase, nitric oxide, arginine decarboxylase, agmatine, agmatinase, arginase activity, neuroinflammation, neurogenesis, immune system, acute ischemic stroke.

中文翻译：

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多胺合成减少在缺血性卒中中的作用

摘要

我们的目标是通过揭示可能的结果来确定急性缺血性中风（AIS）急性和亚急性期精氨酸脱羧酶（ADC），鸟氨酸脱羧酶（ODC）和胍丁胺，鸟氨酸，一氧化氮（NO）和精氨酸酶活性的水平这些水平在神经炎症和神经发生中的作用被假定在AIS之后开始。本研究包括35位被诊断为AIS的患者和35位对照。通过ELISA试剂盒测定ADC，ODC，胍丁胺酶和NO水平。分光光度法测定精氨酸酶活性和鸟氨酸水平。AIS组第1天和第7天的胍丁胺酶，鸟氨酸和精氨酸酶活性水平与对照组相比无统计学差异（p> 0.05）。与对照组相比，急性和亚急性期患者的ADC高，ODC和NO值低（p <0.05）。结果表明，AIS后多胺合成在急性期和亚急性期均下降。这是由于主要途径的速率调节步骤ODC含量降低所致。这种减少可能与神经生成的刺激不足和NO合成减少的免疫系统有关。

关键字：

多胺合成酶，鸟氨酸，鸟氨酸脱羧酶，一氧化氮，精氨酸脱羧酶，胍丁胺，胍丁胺酶，精氨酸酶活性，神经炎症，神经发生，免疫系统，急性缺血性中风。