Calcium carbonate (CaCO₃) is a widely used material in biomedical applications owing to its biocompatibility and widespread occurrence in nature, especially in the exoskeletons of marine organisms. The ability to undergo polymorphic transformation between CaCO₃ polymorphs in aqueous environments provides a wide range of applications in the biomedical field, i.e. vaterite can gradually dissolve to release drugs or calcite can provide rigidity as a bone regeneration material. In this study, CaCO₃ particles including ellipsoidal vaterite, bowknot-like aragonite, and rhombohedral calcite were synthesized via solution mixing method by altering precursor ratio, pH, temperature, and solvent concentration. Afterwards, CaCO₃ particles were interacted with human bone cells (hFOB) to assess cellular proliferation for orthopedic applications. Results showed that hFOB cell viability can be altered by the polymorph and concentration of CaCO₃ particles. As an important finding, while proliferation of hFOB cells was promoted up to 5 days in vitro upon the interaction with vaterite and calcite particles, cellular proliferation was inhibited by aragonite particles at the highest particle concentration. Therefore, this work systematically showed the potential use of different CaCO₃ polymorphs in orthopedic applications.

碳酸钙（CaCO ₃）由于其生物相容性和在自然界中广泛存在，特别是在海洋生物的外骨骼中，是在生物医学应用中被广泛使用的材料。在水性环境中，CaCO ₃多晶型物之间进行多晶型转变的能力在生物医学领域提供了广泛的应用，即球gradually石可以逐渐溶解以释放药物，而方解石可以提供作为骨再生材料的刚性。本研究通过改变前驱体比例，pH，温度和溶剂浓度，通过溶液混合法合成了包括椭圆形球ate石，蝴蝶结状文石和菱形方解石在内的CaCO ₃颗粒。之后，CaCO ₃颗粒与人骨细胞（hFOB）相互作用以评估骨科应用的细胞增殖。结果表明，hFOB细胞的活力可以通过CaCO ₃颗粒的多晶型物和浓度而改变。一个重要发现是，尽管与v石和方解石颗粒相互作用，hFOB细胞的增殖在体外最多可促进5天，但文石颗粒在最高颗粒浓度下抑制了细胞增殖。因此，这项工作系统地显示了在骨科应用中潜在使用不同的CaCO ₃多晶型物的潜力。