This work reports on a rapid diagnostic platform for the detection of Plasmodium falciparum lactate dehydrogenase (PfLDH), a representative malaria biomarker, using a microfluidic microplate-based immunoassay. In this study, the microfluidic microplate made it possible to diagnose PfLDH with a small volume of sample (only 5 μL) and short time (< 90 min) compared to conventional immunoassays such as enzyme-linked immunosorbent assay (ELISA). Moreover, the diagnostic performance of PfLDH showed high sensitivity, specificity, and selectivity (i.e., 0.025 pg/μL in phosphate-buffered saline and 1 pg/μL in human serum). The microfluidic-based microplate sensing platform has the potential to adapt simple, rapid, and accurate diagnoses to the practical detection of malaria.

中文翻译：

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使用基于微流控的恶性疟原虫乳酸脱氢酶免疫分析的简单、快速、准确的疟疾诊断平台


这项工作报告了使用基于微流控微孔板的免疫测定法检测恶性疟原虫乳酸脱氢酶（PfLDH）（一种代表性疟疾生物标志物）的快速诊断平台。在本研究中，与酶联免疫吸附测定（ELISA）等传统免疫测定相比，微流控微孔板可以用少量样品（仅5μL）和短时间（％3C 90分钟）诊断PfLDH。此外，PfLDH 的诊断性能显示出高灵敏度、特异性和选择性（即磷酸盐缓冲盐水中为 0.025 pg/μL，人血清中为 1 pg/μL）。基于微流控的微板传感平台具有使简单、快速、准确的诊断适应疟疾实际检测的潜力。