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Field trial evidence of non-transgenic and transgenic Bt. rice genotypes in north of Iran
Journal of Genetic Engineering and Biotechnology ( IF 3.6 ) Pub Date : 2020-05-01 , DOI: 10.1186/s43141-020-00028-8
Salman Dastan , Behzad Ghareyazie , Shahpour Abdollahi

Field-testing genetically modified crops provides scientists with an opportunity to collect information on environmental interactions and agronomic performance, which is critical to a full environmental safety assessment as required by regulatory authorities. As a result, the goal of this research was field trial of transgenic and conventional rice genotypes. The experiment was carried out in a randomized complete blocks design (RCBD) with four replications and seven genotypes in three isolated regions under the biosafety standard protocol in north of Iran in 2016. In this study, four transgenic lines with an active cry1Ab gene in the vegetative stage and three conventional genotypes (control) as treatment were assessed. The findings demonstrated that in all three regions, transgenic lines derived from Khazar cultivar, were similar to their parent(s) in terms of growth phenology, agronomical traits, grain amylose content, gel consistency, and gelatinization temperature. In all the three regions, the highest number of panicle per m2, number of filled spikelet per panicle, and filled spikelet percentage per panicle were obtained for transgenic lines. Khazar cultivar compared to the transgenic lines showed lower paddy yield. In all the three regions, transgenic lines had lower yield loss than their non-transgenic parent. The lowest number of white heads belonged to transgenic Tarom Molaii and transgenic lines. The most positive direct effect on paddy yield was related to the number of filled spikelet per panicle. Thus, this can be a good trait to achieve higher yield derived from reducing the negative indirect effect of dead heart and white heads. It can be concluded that by producing transgenic rice, which is resistant to stem borer with an active promoter in the reproductive stage, farmers can reduce significant part of yield loss resulting from dead heart and white heads directly correlating with the number of filled spikelet per panicle and paddy yield.

中文翻译:

非转基因和转基因Bt的现场试验证据。伊朗北部的水稻基因型

转基因作物的现场测试为科学家提供了收集有关环境相互作用和农艺性能信息的机会,这对于监管机构要求进行全面的环境安全评估至关重要。结果,本研究的目的是转基因和常规水稻基因型的田间试验。根据2016年伊朗北部生物安全标准规程,该实验以随机完整区组设计(RCBD)进行,在三个隔离区进行了四个复制和七个基因型。在这项研究中,四个转基因品系中带有活跃cry1Ab基因评估了营养阶段和三种常规基因型(对照)作为治疗方法。研究结果表明,在所有三个区域中,源自Khazar品种的转基因品系 在生长物候,农艺性状,谷物直链淀粉含量,凝胶稠度和糊化温度方面与母本相似。在这三个区域中,对于转基因品系,获得了最高的每平方米穗数,每穗实穗数和每穗实穗率。与转基因品系相比,Khazar品种显示出较低的稻谷产量。在这三个区域中,转基因品系的产量损失均低于其非转基因亲本。白头数量最少的是转基因Tarom Molaii和转基因品系。对水稻产量最直接的直接影响与每个穗粒填充的小穗数量有关。因此,这可能是获得较高产量的一个良好特性,因为它可以减少死心和白头的负面间接影响。
更新日期:2020-05-01
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