Pseudomonas aeruginosa is a bacterial pathogen that can cause grave and sometimes chronic infections in patients with weakened immune systems and cystic fibrosis. It is expected that sodium/proton transporters in the cellular membrane are crucial for the organism’s survival and growth under certain conditions, since many cellular processes rely on the maintenance of Na+ and H+ transmembrane gradients. This study focused on the role of the primary and secondary proton and/or sodium pumps Mrp, Nuo, NhaB, NhaP, and NQR for growth, biofilm formation, and swarming motility in P. aeruginosa. Using mutants with gene deletions, we investigated the impact of each sodium pump’s absence on the overall growth, biofilm formation, motility, and weak acid tolerance of the organism. We found that the absence of some, but not all, of the sodium pumps have a deleterious effect on the different phenotypes of P. aeruginosa. The absence of the Mrp sodium/proton antiporter was clearly important in the organism’s ability to survive and function in environments of higher pH and sodium concentrations, while the absence of Complex I, which is encoded by the nuo genes, had some consistent impact on the organism’s growth regardless of the pH and sodium concentration of the environment.

中文翻译：

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在挑战性条件下铜绿假单胞菌的钠反向转运蛋白：对生长，生物膜形成和成群运动的影响

铜绿假单胞菌是一种细菌病原体，可在免疫系统弱和囊性纤维化患者中引起严重的，有时甚至是慢性的感染。可以预期，在某些条件下，细胞膜中的钠/质子转运蛋白对于生物的生存和生长至关重要，因为许多细胞过程都依赖于Na +和H +跨膜梯度的维持。这项研究的重点是初级和次级质子泵和/或钠泵Mrp，Nuo，NhaB，NhaP和NQR对铜绿假单胞菌的生长，生物膜形成和成群运动的作用。使用具有基因缺失的突变体，我们调查了每个钠泵缺失对生物整体生长，生物膜形成，运动性和弱酸耐受性的影响。我们发现缺少一些（但不是全部），的钠泵对铜绿假单胞菌的不同表型具有有害作用。缺少Mrp钠/质子反转运蛋白对于生物体在较高pH和钠浓度的环境中的生存和功能的能力显然很重要，而缺少由nuo基因编码的Complex I对其稳定性产生一定的影响。生物的生长，与环境的pH和钠浓度无关。