The present study aims to apply an efficient eco-friendly and inexpensive process for green synthesis of silver nanoparticles (AgNPs) through the mediation of fungal proteins from Aspergillus fumigatus DSM819, characterization, and its application as antimicrobial finishing agent in textile fabrics against some infectious microorganisms. Optimum conditions for AgNP biosynthesis could be achieved by means of using 60% (v/v) of cell-free filtrate (CFF) and 1.5 mM of AgNO3 at pH 10.0 after 90 min. The obtained AgNPs were of spherical shape with 90% of distribution below than 84.4 nm. The biosynthesized AgNPs exerted an antimicrobial activity against the studied pathogenic microorganisms (E. coli, B. mycoides, and C. albicans). In addition, IC50 values against in vitro tumor cell lines were found to be 31.1, 45.4, 40.9, and 33.5 μg/ml for HCT116, A549, MCF7, and PC3, respectively. Even with a very low concentration (0.25%), the treated PET/C fabrics by AgNPs exerted an antimicrobial activity against E. coli, B. mycoides, and C. albicans to give inhibition zone diameter of 15, 15, and 16 mm, respectively. The green biosynthesis approach applied in this study is a non-toxic alternative to the traditional chemical and physical methods, and would be appropriate for biological large-scale production and prospective treatments.

中文翻译：

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真菌蛋白诱导的银纳米颗粒的生物合成，表征及其在不同生物活性中的应用

本研究旨在通过烟曲霉DSM819的真菌蛋白介导，表征及其作为抗菌整理剂在纺织品中对抗某些感染性微生物的应用，为绿色合成银纳米颗粒（AgNPs）应用一种高效，环保的廉价方法。 。90分钟后，可通过使用60％（v / v）的无细胞滤液（CFF）和1.5 mM的AgNO3（pH 10.0）来实现AgNP生物合成的最佳条件。所获得的AgNP为球形，具有低于84.4nm的90％的分布。生物合成的AgNP对研究的病原微生物（大肠杆菌，B。mycoides和白色念珠菌）具有抗微生物活性。此外，HCT116，A549，HCT116对体外肿瘤细胞株的IC50值分别为31.1、45.4、40.9和33.5μg/ ml。分别为MCF7和PC3。即使使用非常低的浓度（0.25％），经AgNP处理的PET / C织物也可对大肠杆菌，分支杆菌和白色念珠菌发挥抗菌作用，从而形成15、15和16 mm的抑菌圈直径，分别。本研究中采用的绿色生物合成方法是传统化学和物理方法的无毒替代品，适用于生物大规模生产和前瞻性处理。