The current study was conducted to identify the genetic polymorphism of ghrelin (GHRL) gene of sheep and goats, as well as to determine whether these polymorphisms were associated with the evolutionary genetic differences in the involved species. This study was performed on 233 sheep and 91 goats. Two genetic loci of 113 bp and 262 bp partially spanning over exon 2/intron 2 and intron 4/exon 5 of GHRL gene respectively were amplified and genotyped using polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods. The SSCP banding pattern of 262-bp locus indicated the presence of four diplotypes (BC, BB, AC, and AB) in Awassi sheep, three diplotypes (BC, BB, and AB) in Karadi sheep, and only two diplotypes (BC and BB) in all goats’ samples. The current study detected several novel SNPs in the ovine–caprine populations as well as two SNPs that are observed only in sheep, including intron4:119 C>A and intron4:123 T>G. The phylogenetic analysis revealed that the observed diplotypes resided within ovine sequences and were closely related to caprine counterparts. Computational analyses indicated the presence of various intronic RNA motifs. However, all these motifs were gathered in Awassi breed. It is stated that the intron 4 is highly diverse amongst goats and sheep as well as within sheep with a particular emphasis on Awassi. This genetic peculiarity may in turn suggest a high polymorphic pattern of this breed in comparison with other related counterparts.

中文翻译：

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基于GHRL基因的绵羊和山羊品种的基因分型揭示了Awassi绵羊中具有多个RNA图案的高度多态性内含子序列

进行当前的研究以鉴定绵羊和山羊的生长素释放肽（GHRL）基因的遗传多态性，并确定这些多态性是否与所涉及物种的进化遗传差异有关。这项研究是针对233只绵羊和91只山羊进行的。利用聚合酶链反应-单链构象多态性（PCR-SSCP）和DNA测序方法分别扩增了GHRL基因第2外显子2 /内含子2和第4内含子4 /外显子5的113 bp和262 bp的两个基因座，并进行了基因分型。 。262 bp位点的SSCP谱带显示在Awassi绵羊中存在四种双型（BC，BB，AC和AB），在Karadi绵羊中存在三种双型（BC，BB和AB），仅存在两种双型（BC和BB） BB）。当前的研究在绵羊-山羊种群中检测到几种新的SNP，以及仅在绵羊中观察到的两个SNP，包括intron4：119 C> A和intron4：123 T> G。系统发育分析表明，观察到的双倍型存在于绵羊序列中，并且与山羊对应物密切相关。计算分析表明存在各种内含子RNA基序。但是，所有这些图案都是在Awassi品种中收集的。据说内含子4在山羊和绵羊之间以及在绵羊中高度多样化，特别强调Awassi。与其他相关的同类动物相比，这种遗传特性可能反过来暗示了该品种的高多态性模式。系统发育分析表明，观察到的双倍型存在于绵羊序列中，并且与山羊对应物密切相关。计算分析表明存在各种内含子RNA基序。但是，所有这些图案都是在Awassi品种中收集的。据说内含子4在山羊和绵羊之间以及在绵羊中高度多样化，特别强调Awassi。与其他相关的同类相比，这种遗传上的特殊性可能反过来暗示了该品种的高多态性。系统发育分析表明，观察到的双倍型存在于绵羊序列中，并且与山羊对应物密切相关。计算分析表明存在各种内含子RNA基序。但是，所有这些图案都是在Awassi品种中收集的。据说内含子4在山羊和绵羊之间以及在绵羊中高度多样化，特别强调Awassi。与其他相关的同类相比，这种遗传上的特殊性可能反过来暗示了该品种的高多态性。据说内含子4在山羊和绵羊之间以及在绵羊中高度多样化，特别强调Awassi。与其他相关的同类动物相比，这种遗传特性可能反过来暗示了该品种的高多态性模式。据说内含子4在山羊和绵羊之间以及在绵羊中高度多样化，特别强调Awassi。与其他相关的同类动物相比，这种遗传特性可能反过来暗示了该品种的高多态性模式。