The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.

中文翻译：

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体外再生方法来恢复莫氏罗汉果（Ceropegia mohanramii）-一种地方病和极度濒危的马齿pi

这项研究旨在开发一种高效，快速，大规模的体外再生系统，以繁殖，保存和恢复一种特有和极度濒危的草药Ceropegia mohanramii。使用节点外植体在补充了6-苄基氨基嘌呤（BAP：1.0 mg / l）的Murashige和Skoog's（MS）培养基上建立培养物。在补充了BAP（2.0 mg / l）和吲哚-3-丁酸（IBA，0.5 mg / l）的MS培养基上培养的结节芽在100％的培养物中产生最大芽数（17.1±1.2） 。补充BAP（2.0 mg / l）的MS培养基以及不同浓度的吲哚3乙酸（IAA）促进了体外开花。将体外再生的芽转移至用单数补充生长素强化的二分之一MS培养基中，其中IBA（1。5 mg / l）最适合最大根数（5.7±0.6）的生产。将体外衍生的小植株在温室中的受控条件下硬化，然后转移到土壤中。超过1200棵树苗被移植到西高止山脉的八个不同地区，在移植1年后，存活率达到76％以上。