In this work, wheat straw (WS) was used as a lignocellulosic substrate to investigate the influence of pH on enzymatic saccharification. The optimum enzymatic hydrolysis occurred at pH range 5.8–6.0, instead of 4.8–5.0 as has been widely reported in research. Two enzymes cocktails, Celluclast® 1.5 L with Novozymes 188, Cellic® CTec2 and endo-1,4-β-xylanase, were used for the pH investigation over a pH range of 3.0–7.0. The highest concentration of total reduced sugar was found at pH 6.0 for all the different enzymes used in this study. The total reduced sugar produced from the enzymatic saccharification at pH 6.0 was found to be 7.0, 7.4, and 10.8 (g L −1 ) for Celluclast® 1.5 L with Novozymes 188, endo-1,4-β-xylanase and Cellic® CTec2, respectively. By increasing the pH from 4.8 to 6.0, the total reduced sugar yield increased by 25% for Celluclast® 1.5 L with Novozymes 188 and endo-1 4-β-xylanase and 21% for Cellic® CTec2. The results from this study indicate that WS hydrolysis can be improved significantly by elevating the pH at which the reaction occurs to the range of 5.8 to 6.0.

中文翻译：

---

优化 pH 作为改善小麦秸秆酶促糖化以提高生物乙醇产量的策略

在这项工作中，小麦秸秆 (WS) 用作木质纤维素底物，以研究 pH 值对酶促糖化的影响。最佳酶水解发生在 pH 值范围 5.8-6.0，而不是研究中广泛报道的 4.8-5.0。两种酶混合物，即 Celluclast® 1.5 L 和 Novozymes 188、Cellic® CTec2 和内切-1,4-β-木聚糖酶，用于在 3.0–7.0 的 pH 范围内进行 pH 研究。对于本研究中使用的所有不同酶，在 pH 6.0 时发现总还原糖的最高浓度。对于 Celluclast® 1.5 L 和 Novozymes 188、内切-1,4-β-木聚糖酶和 Cellic® CTec2，发现在 pH 6.0 的酶促糖化产生的总还原糖为 7.0、7.4 和 10.8 (g L -1 ) ， 分别。通过将 pH 从 4.8 增加到 6.0，使用 Novozymes 188 和内切 1 4-β-木聚糖酶的 Celluclast® 1.5 L 的总还原糖产量增加了 25%，而 Cellic® CTec2 的总还原糖产量增加了 21%。这项研究的结果表明，通过将反应发生的 pH 值提高到 5.8 到 6.0 的范围，可以显着改善 WS 水解。