The present study investigated the anti-Trypanosoma brucei brucei activity of methanolic extract of Senna occidentalis roots, leaves, stem bark, and seed in vitro and bioactive components of the most active plant part. Trypanosoma brucei brucei was identified by PCR, cultured in Dulbecco’s Modified Eagle Medium (DMEM) and incubated at 37 °C and 5% CO2. Subsequently, the cultured T. brucei brucei were incubated with 500 μg/ml concentration of different parts of S. occidentalis plant and the most potent fraction was identified and subjected to Gas Chromatography-Mass Spectrometry (GC-MS). Amplicons of the rRNA gene of T. brucei brucei detected had a size of 1200 bp. The anti-trypanosomal activities indicated that the root extract of the plant was the most active at 500 μg/ml with inhibitory activity of 88.89% and fractions (2 and 5) the most active with IC50 values of 1.49 and 1.16 μg/ml respectively. Results from this study insinuates that phenolic and simple aliphatic compounds might play key role in the anti-trypanosomal activity of S. occidentalis roots.

中文翻译：

---

布鲁氏锥虫的分子鉴定和西番莲甲醇提取物不同部位的体外抗锥虫活性

本研究调查了西番莲根，叶，茎皮和种子的甲醇提取物在体外的抗锥虫布鲁氏菌活性以及植物中活性最高的部分的生物活性成分。通过PCR鉴定出布鲁氏锥虫，在Dulbecco改良Eagle培养基（DMEM）中培养并在37℃和5％CO 2下孵育。随后，将培养的布鲁氏布鲁氏菌与500μg/ ml浓度的西方链球菌植物的不同部分孵育，并鉴定出最有效的馏分，并进行气相色谱-质谱分析（GC-MS）。所检测到的布鲁氏布鲁氏菌rRNA基因的扩增子大小为1200 bp。抗锥虫活性表明该植物的根提取物以500μg/ ml的活性最高，抑制活性为88。89％和馏分（2和5）最有活性，IC50值分别为1.49和1.16μg/ ml。这项研究的结果表明，酚类和简单的脂族化合物可能在西方葡萄球菌根的抗锥虫活性中起关键作用。