The stem bark aqueous alcohol extract of Poincianella pluviosa (PPSB extract) is rich in bioactives including gallic acid, gallic acid methyl ester, pyrogallol, ellagic acid, corilagin, 1,4,6-tri-O-galloyl-glucose, 1,2,3,6-tetra-O-galloyl-glucose, 1,2,3,4,6-penta-O-galloyl-glucose, tellimagrandin I, tellimagrandin II, mallotinic acid, mallotusinic acid, and geraniin. The aim of the present study was to evaluate the antioxidant activity of the PPSB extract as well as its inhibitory action on carbohydrate digestive enzymes relevant to type 2 diabetes. The PPSB extract was prepared using a mixture of 40% ethanol and 60% distilled water. The PPSB extract showed high antioxidant activities and inhibited several carbohydrate digestive enzymes. The IC50 values for inhibiting in vitro salivary amylase, pancreatic amylase, intestinal β-galactosidase and intestinal invertase were, respectively, 250 ± 15, 750 ± 40, 25 ± 5, and 75 ± 8 μg/mL. In vivo inhibition of the intestinal starch absorption was confirmed by determination of blood glucose levels in rats before and after administration of starch by gavage with or without different amounts of PPSB extract. Docking simulations performed on three different programs to rank the extract compounds most likely to bind to porcine pancreatic α-amylase suggest that geraniin is likely to be the P. pluviosa extract compound that presents the greatest binding potential to the pancreatic alpha-amylase. However, the total inhibitory action of the PPSB extract is likely to result from a summation of effects of several molecules. Furthermore, the PPSB extract did not present acute toxicity nor did it present mutagenic effects. It can be concluded that the PPSB extract is potentially useful in controlling the postprandial glycaemic levels in diabetes. Further clinical studies with the extract are needed, however, to confirm its potential use in the management of type 2 diabetes.

中文翻译：

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Poincianella pluviosa茎皮提取物可抑制碳水化合物消化酶：与2型糖尿病治疗的相关性

inc药的茎皮含水醇提取物（PPSB提取物）富含生物活性物质，包括没食子酸，没食子酸甲酯，邻苯三酚，鞣花酸，可乐菌素，1,4,6-三-O-没食子酰基-葡萄糖，1,2 1,3,6-四-O-没食子酰基葡萄糖，1,2,3,4,6-戊五-O-没食子酰基葡萄糖，tellimagrandin I，tellimagrandin II，Mallotinic acid，Mallotusinic acid和geraniin。本研究的目的是评估PPSB提取物的抗氧化活性及其对与2型糖尿病相关的碳水化合物消化酶的抑制作用。使用40％乙醇和60％蒸馏水的混合物制备PPSB提取物。PPSB提取物显示出高抗氧化活性，并抑制了几种碳水化合物的消化酶。抑制体外唾液淀粉酶，胰淀粉酶，肠道β-半乳糖苷酶和肠道转化酶分别为250±15、750±40、25±5和75±8μg/ mL。通过在用或不用不同量的PPSB提取物进行管饲法施用淀粉之前和之后测定大鼠中的血糖水平来证实肠内淀粉吸收的体内抑制。在三个不同程序上进行的对接模拟对最可能与猪胰腺α-淀粉酶结合的提取物化合物进行排名，表明香叶素可能是P. pluviosa提取物化合物，对胰腺α-淀粉酶具有最大的结合潜力。但是，PPSB提取物的总抑制作用可能是几种分子作用的总和。此外，PPSB提取物既不表现出急性毒性也不表现出诱变作用。可以得出结论，PPSB提取物可用于控制糖尿病的餐后血糖水平。然而，需要对提取物进行进一步的临床研究，以确认其在治疗2型糖尿病中的潜在用途。