Enantia chlorantha is a local medicinal plant commonly use in Nigeria for the treatment of diabetes but without support of scientific data. Large percentage of people suffering from diabetes who uses the plant as antidiabetic agent also combine its administration with standard antihypertensive drugs. In the present study, we have investigated the possible toxicological effects of combined administration of E. chlorantha bark extract and lisinopril in diabetic model of experimental rats. E. chlorantha stem bark was extracted by cold maceration of the pulverised stem bark in 70% ethanol. The acute toxicity effect of the plant was then evaluated in rats following oral administration of single dose of the extract. Diabetes was induced by intraperitoneal administration of 40 mg/kg streptozotocin into fructose fed rat. Diabetic rats were then randomly assigned into 6 groups of 7 rats each. One group was kept as the diabetic model while separate treatments were administered to the other six groups. Seven non diabetic rats were kept as the control group and administered normal saline. The LD50 of E. chlorantha stem bark was above 5000 mg/kg. Combined administration of lisinopril and E. chlorantha showed synergistic effects in the restoration of renal biomarkers (serum creatinine, urea, Na+ and K+), cardiac function biomarkers (CK-MB and LDH) and hematological parameters (RBC, WBC, HGB and PCV), while antagonistic effects were however observed with some of the liver biomarkers (AST, ALT, ALP, GGT, total protein and total bilirubin). Rats co-administered lisinopril and E. chlorantha also showed fatty liver with cholestasis. The study concluded that diabetes is associated with kidney and cardiac dysfunction. Combined administration of lisinopril and E. chlorantha though may not aggravate these dysfunctions however, it may antagonize the efficacy of the plant in ameliorating liver dysfunction in diabetics.

中文翻译：

---

百草枯和赖诺普利乙醇提取物对实验2型糖尿病的毒理学评价。

Enantia chlorantha是尼日利亚的一种当地药用植物，通常用于治疗糖尿病，但没有科学数据的支持。使用植物作为抗糖尿病药的糖尿病患者中，有很大一部分人也将其与标准降压药合用。在本研究中，我们研究了将绿皮草皮提取物和赖诺普利合用在实验大鼠糖尿病模型中的可能的毒理作用。通过在70％的乙醇中冷浸粉碎的茎皮，提取出绿叶桉茎皮。然后在口服给予单剂量提取物后，在大鼠中评估了植物的急性毒性作用。通过腹膜内给果糖喂养的大鼠腹膜内施用40 mg / kg链脲佐菌素来诱导糖尿病。然后将糖尿病大鼠随机分为6组，每组7只。将一组作为糖尿病模型，而对其他六组进行单独的治疗。保留7只非糖尿病大鼠作为对照组并给予生理盐水。绿藻大肠干皮的LD50大于5000 mg / kg。赖诺普利和绿藻的联合给药在肾脏生物标志物（血清肌酐，尿素，Na +和K +），心脏功能生物标志物（CK-MB和LDH）和血液学参数（RBC，WBC，HGB和PCV）的恢复中显示协同作用。 ，但是对某些肝脏生物标志物（AST，ALT，ALP，GGT，总蛋白和总胆红素）却观察到了拮抗作用。共同使用赖诺普利和绿藻的大鼠也表现出脂肪肝和胆汁淤积。研究得出结论，糖尿病与肾脏和心脏功能障碍有关。赖诺普利和绿藻的联合给药虽然可能不会加剧这些功能障碍，但是，它可能拮抗植物改善糖尿病患者肝脏功能障碍的功效。