The current study undertaken to evaluate antioxidant property of Solanum macranthum fruit methanol extract and its in-vitro cytotoxic effects on breast cancer MDA-MB-231 and mice embryo fibroblast (MEF-L929) cell line. The total alkaloids, phenols and flavonoids content in the methanol extract of S.macranthum fruit were estimated. The antioxidant activity was evaluated by FRAP assay, H2O2 scavenging assay and DPPH assay. Anti-inflammatory protein denaturation inhibition assay was carried out using bovine serum albumin. Anticancer property of the extract was evaluated against breast cancer MDA-MB-231 by MTT assay and comparative study was carried out on normal cells using MEF-L929 cell line. Phytochemical estimation revealed that total alkaloid content was measurable high (13.6 g/100 g of extract), where as the total phenolics and flavonoids content was 115.5 mg/GAE and 142.6 mg/QE. Further, the antioxidant effect of methanol extract by H2O2 assay showed considerably higher scavenging activity with 99.6% at 400 μg/ml, where as by FRAP assay the relative reducing power was estimated to be 39.1% at 400 μg/ml and in case of DPPH assay it was found to be 47.9% at 400 μg/ml. EC50 value of anti-inflammatory assay was found to be 112.96 μg/ml. Anticancer studies showed that the IC50 value was found to be 373.77 μg /ml against MDA-MB-231 breast cancer cell line, where as the methanol extract showed negligible cytotoxic effect against normal cells (MEF -L929 cell line), indicating its cancer specific effect. Based on the results obtained, methanol extract of Solanum macranthum fruit was found to possess significant antioxidant properties and also showed potential anticancer property against MDA-MB-231 breast cancer cell line with negligible cytotoxic effect on normal cells revealing its potential application as an anticancer agent.

中文翻译：

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体外细胞毒性的影响茄macranthum果。具有抗氧化潜能的杜南提取物

当前的研究旨在评估茄果甲醇提取物的抗氧化性能及其对乳腺癌MDA-MB-231和小鼠胚胎成纤维细胞（MEF-L929）细胞系的体外细胞毒性作用。估算了大麦果实甲醇提取物中总生物碱，酚和类黄酮的含量。抗氧化剂活性通过FRAP测定，H 2 O 2清除测定和DPPH测定进行评估。使用牛血清白蛋白进行抗炎蛋白变性抑制测定。通过MTT分析评估提取物对乳腺癌MDA-MB-231的抗癌特性，并使用MEF-L929细胞系对正常细胞进行比较研究。植物化学估计表明，总生物碱含量很高（13.6 g / 100 g提取物），其中总酚和类黄酮含量分别为115.5 mg / GAE和142.6 mg / QE。此外，H2O2测定法对甲醇提取物的抗氧化作用显示出相当高的清除活性，在400μg/ ml时为99.6％，其中，通过FRAP测定法，在400μg/ ml时，相对还原能力估计为39.1％，在DPPH的情况下分析发现，在400μg/ ml下为47.9％。发现抗炎测定的EC 50值为112.96μg/ ml。抗癌研究表明，针对MDA-MB-231乳腺癌细胞系的IC50值为373.77μg/ ml，其中甲醇提取物对正常细胞（MEF -L929细胞系）的细胞毒性作用可忽略不计，表明其对癌症的特异性影响。根据获得的结果，