Hydroxamic acids are currently being used in diverse biological activities. We investigated the effect of hydroxamic acid, synthesized from Cyperus esculentus seeds, on the antioxidant status of the liver, spleen, and kidney of Wistar rats. Twenty male rats were randomly divided into three treatment groups using hydroxamic acid at doses of 5, 15, and 50 mg/kg and a control group using distilled water. Rats were sacrificed 24 h after a seven-day repeated oral dosing. After that, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and the levels of glutathione (GSH) and malondialdehyde (MDA) were investigated in the organs. Our data showed that MDA levels remained unaltered in the liver, spleen, and kidney. However, it was found that hydroxamic acid at the dose of 50 mg/kg significantly increased SOD activity but depleted CAT, GPx activities and GSH levels in the liver when compared to the control groups. In splenic tissue, SOD activity and GSH levels were significantly diminished. Contrarily, in the kidney, treatment of rats with 50 mg/kg hydroxamic acid did not affect SOD activity, but GPx activity was increased while GST activity was decreased when compared to the controls. Overall, hydroxamic acid may enhance antioxidant enzyme activities in the liver and kidney. However, caution is required at higher doses to forestall oxidative stress in the hepatic and splenic tissues.

中文翻译：

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高剂量绿色合成的异羟肟酸可破坏白化病大鼠肝和脾组织中的抗氧化剂平衡

异羟肟酸目前用于多种生物活动中。我们研究了从香附子种子合成的异羟肟酸对Wistar大鼠肝脏，脾脏和肾脏抗氧化状态的影响。将20只雄性大鼠随机分为3个治疗组，分别使用剂量为5、15和50 mg / kg的异羟肟酸，对照组为使用蒸馏水的对照组。重复口服7天后24小时，处死大鼠。之后，研究了器官中的超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx），谷胱甘肽S-转移酶（GST）和谷胱甘肽（GSH）和丙二醛（MDA）的活性。 。我们的数据显示，肝脏，脾脏和肾脏中的MDA水平保持不变。然而，与对照组相比，发现50 mg / kg剂量的异羟肟酸显着增加了SOD活性，但减少了肝脏中的CAT，GPx活性和GSH水平。在脾脏组织中，SOD活性和GSH水平明显降低。相反，在肾脏中，与对照组相比，用50 mg / kg异羟肟酸处理大鼠不会影响SOD活性，但GPx活性增加而GST活性降低。总体而言，异羟肟酸可能会增强肝脏和肾脏中的抗氧化酶活性。但是，在高剂量时需要谨慎，以防止肝和脾组织中的氧化应激。SOD活性和GSH水平显着降低。相反，在肾脏中，与对照组相比，用50 mg / kg异羟肟酸处理大鼠不会影响SOD活性，但GPx活性增加而GST活性降低。总体而言，异羟肟酸可能会增强肝脏和肾脏中的抗氧化酶活性。但是，在高剂量时需要谨慎，以防止肝和脾组织中的氧化应激。SOD活性和GSH水平显着降低。相反，在肾脏中，与对照组相比，用50 mg / kg异羟肟酸处理大鼠不会影响SOD活性，但GPx活性增加而GST活性降低。总体而言，异羟肟酸可能会增强肝脏和肾脏中的抗氧化酶活性。但是，在高剂量时需要谨慎，以防止肝和脾组织中的氧化应激。