In this present study, antioxidant, anti-hemolytic, cytotoxic and anti-bacterial activities derived from methanol extracts of Bruguiera gymnorrhiza and Heritiera littoralis were investigated in order to determine their medicinal activities. Folin-Ciocalteu reagent method and aluminum chloride methods were used to determine the mangroves’ total phenolics and total flavonoid content, respectively. Antioxidant capacity was assessed via the following methods: 1,1-diphenyl-2-picryl hydroxyl (DPPH), 43 mM H2O2, Fe2+ quenching assay, and anti-hemolytic activity. Brine shrimp (Artemia salina L.) lethality assay was also carried out to determine the cytotoxic potential of the mangroves along with anti-bacterial activity test using five Gram-negative and another two Gram-positive bacterial strains. The mangroves yielded 58.917 ± 0.601 and 36.625 ± 0.551 mg Gallic acid equivalent (GAE)/g sample and 76.417 ± 0.19 and 113.637 ± 0.17 mg quercetin equivalent (QE)/g sample in B. gymnorrhiza and H. littoralis, respectively. Methanol extracts of both mangroves exhibited high radical scavenging activity against DPPH, H2O2 and Fe2+ radicals. The reductive capacity of the extracts increased with increasing concentrations of samples, and the extracts inhibited H2O2 induced hemolysis in human red blood cells (RBCs). Antioxidant properties were found to be moderately weaker than that of the reference standard, L-ascorbic acid (AA), and Gallic acid (GA). Further, brine shrimp (Artemia salina L.) lethality assay revealed significant cytotoxicity (241.4 and 272.6 μg/mL, respectively). Methanol extracts could also inhibit the growth of pathogenic bacterial strains. This study showed that the crude methanol extract of selected mangrove plants possesses free radical scavenging, anti-hemolytic, cytotoxic and anti-bacterial activity. The experimented plant has the potential to be used as a traditional medicine and replace synthetic drugs. Further studies are necessary to isolate active compounds responsible for the overall antioxidant activity of the crude extracts.

中文翻译：

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孟加拉国某些红树林植物（Bruguiera Gymnorrhiza和Heritiera littoralis）的抗氧化，抗溶血，细胞毒性作用和抗菌活性的评估

在本研究中，为了确定其药理活性，对甘草假单胞菌和滨海heritiera甲醇提取物的抗氧化，抗溶血，细胞毒性和抗菌活性进行了研究。用Folin-Ciocalteu试剂法和氯化铝法分别测定红树林的总酚和总黄酮含量。通过以下方法评估抗氧化能力：1,1-二苯基-2-吡啶基羟基（DPPH），43 mM H2O2，Fe2 +猝灭测定和抗溶血活性。还使用五种革兰氏阴性和另外两种革兰氏阳性细菌菌株进行了盐水虾（卤虫虾）致死率测定，以确定红树林的细胞毒性潜力以及抗菌活性测试。红树林的产量为58.917±0.601和36.625±0。裸子叶杆菌和滨海双歧杆菌分别为551 mg没食子酸当量（GAE）/ g样品和76.417±0.19和113.637±0.17 mg槲皮素当量（QE）/ g样品。两种红树林的甲醇提取物均表现出对DPPH，H2O2和Fe2 +自由基的高自由基清除活性。提取物的还原能力随样品浓度的增加而增加，并且提取物抑制H2O2诱导的人红细胞（RBC）溶血。发现抗氧化特性比参考标准品L-抗坏血酸（AA）和没食子酸（GA）的弱。此外，盐水虾（盐卤虾）的致死率测定显示出显着的细胞毒性（分别为241.4和272.6μg/ mL）。甲醇提取物还可以抑制病原细菌菌株的生长。该研究表明，所选红树林植物的粗甲醇提取物具有自由基清除，抗溶血，细胞毒性和抗菌活性。实验过的植物有可能被用作传统药物并替代合成药物。需要进一步研究以分离出负责粗提物总体抗氧化活性的活性化合物。