A significant role in the pathogenesis of rhinosinusitis is played by an inflammatory reaction in the maxillary sinuses of the nasal cavity. Leukotrienes are the most powerful mediators of inflammation, especially at the early stages. The effect of combined dry extract BNO 1016 could be useful for the inhibition of the inflammation in rhinosinusitis. Thus, it appears reasonable to study the anti-inflammatory activity of the combined dry extract BNO 1016. The tested drug is the combined dry extract BNO 1016 produced by “Bionorica SE”. The reference drug is Ibuprofen. Leukotriene inflammation was induced by subplantar injection of zymozan into the right hind paw of male and female Wistar rats. Volumes of the resulting edema were measured and levels of anti-inflammatory activity together with mean effective dose (ED50) were calculated. The highest anti-inflammatory activity was observed at a dose level of 500 mg/kg of BNO 1016 during all time-points of the experiment and reached 65.2% 2 h after induction of inflammation. The anti-inflammatory activity of BNO 1016 at 500 mg/kg was credibly higher than that of Ibuprofen at all time-points of the experiment. Based on the data obtained from this leukotriene-dependent inflammation experiment the mean effective dose (ED50) for anti-inflammatory activity of dry combined extract BNO 1016 was calculated with help of Probit analysis. The combined dry extract BNO 1016 shows a high level of anti-inflammatory activity in leukotriene-dependent inflammation which is very promising for the improvement of the treatment of patients. However, additional preclinical and clinical research on this drug should be carried out.

中文翻译：

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联合提取物BNO 1016在白三烯依赖性体内炎症模型中的抗炎作用研究

在鼻-鼻窦炎的发病机理中的重要作用是鼻腔上颌窦的炎症反应。白三烯是炎症最强大的介质，尤其是在早期。组合的干提取物BNO 1016的作用可能对抑制鼻-鼻窦炎的炎症有用。因此，研究组合的干提取物BNO 1016的抗炎活性似乎是合理的。受试药物是“ Bionorica SE”生产的组合的干提取物BNO 1016。参考药物是布洛芬。通过将zymozan足底注射到雄性和雌性Wistar大鼠的右后爪中诱发白三烯炎症。测量产生的水肿的体积，并计算抗炎活性水平以及平均有效剂量（ED50）。在实验的所有时间点，在500 mg / kg BNO 1016的剂量水平下观察到最高的抗炎活性，并在诱导炎症后2小时达到65.2％。在实验的所有时间点，BNO 1016的500 mg / kg的抗炎活性均明显高于布洛芬。基于从该白三烯依赖性炎症实验获得的数据，借助Probit分析计算出干燥的联合提取物BNO 1016的抗炎活性的平均有效剂量（ED50）。组合的干提取物BNO 1016在白三烯依赖性炎症中显示出高水平的抗炎活性，这对于改善患者的治疗非常有希望。但是，应该对该药物进行更多的临床前和临床研究。