A new prototype of bio-conditioner useful in rehabilitation of degraded soils was performed. In order to obtain this aim two stages were established: production of biomass of Microbacterium sp. CSB3 and formulation of this inoculum in a sediment supplemented with low-rank coal (LRC). The effect of agitation and pH on microbial growth was determined. As response variables, the final production of biomass (Xf) and yield (Yx/s) were determined. Growth dynamics of CSB3 in a 2-L reactor was also evaluated through Xf, Yx/s and the determination of kinetic parameters (specific growth rate [μ] and duplication time [Dt]). The formulation of CSB3 was evaluated; mixtures of several LRC proportions with a sediment from a municipal aqueduct were made. During 90 days, the viability of CSB3 was monitored by counting CFU. The optimal pH and agitation for Xf and Yx/x were 7.5 and 232 rpm, respectively; the values of Xf, Yx/s, μ and Dt in 2-L reactor were: 1.5 gL−1, 0.28 g/g, 0.0208 h−1, 33.3 h, respectively. Regarding the formulation, the most suitable combination to conserve the viability of CSB3 was LRC 25%–sediment 75%; the heavy metals content of LRC allow to infer that the prototype of bio-conditioner does not represent a pollution risk for environment soil. It was possible to optimize the growth of CSB3 under laboratory conditions. The viability of CSB3 could be maintained by a formulation in a sediment supplemented with lignite; this formulation constitutes a new prototype of soil bio-conditioner.

中文翻译：

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用于处理退化土壤的生物调节剂原型的设计：微生物菌种的生物量生产和褐煤配方。CSB3

进行了可用于降解土壤修复的生物调节剂的新原型。为了达到该目的，建立了两个阶段：微生物杆菌属的生物质的生产。CSB3和这种接种物的配方，该沉淀物中添加了低等煤（LRC）。确定了搅拌和pH对微生物生长的影响。作为响应变量，确定了生物质的最终产量（Xf）和产量（Yx / s）。还通过Xf，Yx / s和动力学参数（比生长速率[μ]和复制时间[Dt]）的确定来评估CSB3在2-L反应器中的生长动力学。评价了CSB3的配方；混合了几种LRC比例和市政输水管道的沉淀物。在90天内，通过计算CFU监测CSB3的生存能力。Xf和Yx / x的最佳pH和搅拌分别为7.5和232 rpm。2-L反应器中Xf，Yx / s，μ和Dt的值分别为：1.5 gL-1、0.28 g / g，0.0208 h-1、33.3 h。关于配方，要保持CSB3的活力，最合适的组合是LRC 25％-沉积物75％。LRC中的重金属含量可以推断出，生物调节剂的原型并不代表对环境土壤的污染风险。在实验室条件下可以优化CSB3的生长。CSB3的生存能力可以通过在添加褐煤的沉淀物中进行配制来保持；该配方构成了土壤生物调节剂的新原型。关于配方，要保持CSB3的活力，最合适的组合是LRC 25％-沉积物75％。LRC中的重金属含量可以推断出，生物调节剂的原型并不代表对环境土壤的污染风险。在实验室条件下可以优化CSB3的生长。CSB3的生存能力可以通过在添加褐煤的沉淀物中进行配制来保持。该配方构成了土壤生物调节剂的新原型。关于配方，要保持CSB3的活力，最合适的组合是LRC 25％-沉积物75％。LRC中的重金属含量可以推断出，生物调节剂的原型并不代表对环境土壤的污染风险。在实验室条件下可以优化CSB3的生长。CSB3的生存能力可以通过在添加褐煤的沉淀物中进行配制来保持。该配方构成了土壤生物调节剂的新原型。CSB3的生存能力可以通过在添加褐煤的沉淀物中进行配制来保持。该配方构成了土壤生物调节剂的新原型。CSB3的生存能力可以通过在添加褐煤的沉淀物中进行配制来保持；该配方构成了土壤生物调节剂的新原型。