The present study identified, quantified, and characterized the combinations of phytocomponents from fractionated leaf extracts of Anacardium occidentale, Psidium guajava, and Terminalia catappa that stabilized sickle erythrocyte membrane against osmotic stress, or otherwise, using standard chromatographic-spectrophotometric techniques, namely GC-MS, FT-IR, and UV-visible systems. The percentage hemolysis of the control sample, in 0.9 g/100 mL NaCl, was 35.08 ± 11.64%, whereas those of the samples containing 40 mg/100 mL, 60 mg/100 mL, and 80 mg/100 mL of ethylacetate extracts of T. catappa ranged between 31.82 ± 8.32 and 39.18 ± 6.94%. Ethylacetate extract of T. catappa contained comparative high quantities of hexadecanoic acid methyl ester, 9,11-octadecadienoic acid, methyl ester, (E, E)-, trans-13-octadecenoic acid methyl ester, and methyl stearate. FT-IR and UV-visible spectra showed that ethylacetate extract of T. catappa contained aromatic compounds as well as nitro-compounds, phenolics, and esters. To mention but a few, the combinations of major phytocomponents that stabilized sickle erythrocyte membrane against osmotic stress were hexadecanoic acid, methyl ester, 11-octadecenoic acid, methyl ester, dibutyl phthalate, pentacosane, trans-13-octadecenoic acid, methyl ester, whereas the minor phytocomponents include methyl tetradecanoate, methoxyacetic acid, 3-pentadecyl ester, methyl stearate, hexadecanoic acid, isoxazole, 4,5-dimethyl-.

中文翻译：

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来自 Anacardium occidentale、Psidium guajava 和 Terminalia catappa 的植物成分改变镰状红细胞的膜渗透稳定性

本研究使用标准色谱-分光光度计技术，即 GC-MS，鉴定、量化和表征了来自 Anacardium occidentale、Psidium guajava 和 Terminalia catappa 的分馏叶提取物的植物成分组合，这些植物成分可以稳定镰状红细胞膜以抵抗渗透胁迫或其他、FT-IR 和紫外-可见系统。对照样品在 0.9 g/100 mL NaCl 中的溶血百分比为 35.08 ± 11.64%，而含有 40 mg/100 mL、60 mg/100 mL 和 80 mg/100 mL 乙酸乙酯提取物的样品的溶血百分比为T. catappa 介于 31.82 ± 8.32 和 39.18 ± 6.94% 之间。T. catappa 的乙酸乙酯提取物含有相对大量的十六烷酸甲酯、9,11-十八碳二烯酸甲酯、(E、E)-、反式-13-十八碳烯酸甲酯、和硬脂酸甲酯。FT-IR 和紫外-可见光谱表明 T. catappa 的乙酸乙酯提取物含有芳香族化合物以及硝基化合物、酚类和酯类。仅举几例，稳定镰状红细胞膜抵抗渗透胁迫的主要植物成分的组合是十六烷酸、甲酯、11-十八碳烯酸、甲酯、邻苯二甲酸二丁酯、五十二烷、反式 13-十八碳烯酸、甲酯，而次要植物成分包括十四酸甲酯、甲氧基乙酸、3-十五烷基酯、硬脂酸甲酯、十六烷酸、异恶唑、4,5-二甲基-。