Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants.

中文翻译：

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用于植物扫描电子显微镜的甲醇固定

用于扫描电子显微镜 (SEM) 的植物标本通常使用标准协议进行处理。常规固定剂由有毒化学物质组成，如戊二醛、多聚甲醛和四氧化锇。1996 年，据报道甲醇固定是标准方案的快速替代方案。如果样品在甲醇中浸泡 30 秒或更长时间并在临界点干燥，它们的保存质量似乎与用化学固定剂处理的样品相当。由甲醇固定和乙醇脱水组成的修改版本可有效保留组织形态和尺寸。这些基于溶剂的固定和脱水协议被认为是植物 SEM 标准协议的快速和简单的替代方案。