The objective of this study was to investigate the effects of different enzymolysis conditions on the NO inhibition rate and DH (degree of hydrolysis) of sturgeon hydrolysates (SH) prepared by Alcalase. The NO inhibition rate of 60.23% was attained under the optimum enzymolysis conditions as follows: pH 9.0, enzymolysis time of 4.92 h, enzymolysis temperature of 55°C, solid/liquid ratio of 1 : 20, and enzyme additive amount of 7674.22 U/g protein, which was well matched with the predicted value 61.44% of the Box–Behnken design model. After the ultrafiltration of SH, SH-3 (SH < 3 kDa) could significantly decrease the levels of NO and proinflammatory cytokine level IL-6. Also, we found that the obtained SH-3 contained good properties of emulsification and possessed good WHC and OHC. SH-3 demonstrated appreciable antioxidant potential on DPPH and ABTS radical scavenging activities. These results suggested that SH-3 derived from sturgeon muscles could potentially be used as a promising ingredient against inflammatory and oxidative stress-associated diseases.

中文翻译：

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鲟鱼肌肉酶促条件的优化及其抗炎作用

本研究的目的是研究不同酶解条件对 Alcalase 制备的鲟鱼水解物 (SH) 的 NO 抑制率和 DH（水解度）的影响。最佳酶解条件为：pH 9.0、酶解时间4.92 h、酶解温度55℃、固液比1:20、酶添加量7674.22 U/ g 蛋白，这与 Box-Behnken 设计模型的预测值 61.44% 很好地匹配。SH超滤后，SH-3（SH < 3 kDa）可显着降低NO水平和促炎细胞因子水平IL-6。同时，我们发现得到的SH-3具有良好的乳化性能和良好的WHC和OHC。SH-3 对 DPPH 和 ABTS 自由基清除活性显示出明显的抗氧化潜力。这些结果表明，源自鲟鱼肌肉的 SH-3 可能被用作对抗炎症和氧化应激相关疾病的有前途的成分。