The interest in the therapeutic use of probiotic microorganisms has been increased during the last decade although the doubts have ascended about the probiotics mainly because their beneficial effects are not fully understood, and, in many cases, their usefulness has not been validated in clinical trials. Consequently, the notion got a considerable interest in those strains having proven probiotic potential to be engineered for improvement in their beneficial features. The process of genetic engineering can also be used for probiotic strains for the reversion of antimicrobial resistance and other modifications for their safer and effective human applications. The lactic acid bacilli are predominantly opposite as they already have gained attention owing to their health-promoting benefits and their safety for human consumption; therefore, their use, especially as a delivery agent of vaccines and drugs, is gaining attention. The tailoring of probiotic strains will not only improve the data regarding the probiotic potential of these strains but also clinch the doubts concerning these probiotics. This article focuses on the approaches of bioengineered probiotics and discusses the potential prospects for their therapeutic applications including immunomodulation, cognitive health, and anticancer therapeutics.

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基因工程益生菌的治疗意义的前景

在过去的十年中，人们对益生菌微生物的治疗用途的兴趣有所增加，尽管对益生菌的怀疑有所增加，主要是因为它们的有益作用尚未完全了解，并且在许多情况下，它们的有用性尚未在临床试验中得到验证。因此，这一概念对那些已被证明具有益生菌潜力的菌株产生了相当大的兴趣，可以对其进行工程改造以改善其有益特性。基因工程的过程也可用于益生菌菌株，以逆转抗菌素耐药性和其他修饰，以使其更安全有效地应用于人类。乳酸杆菌主要是相反的，因为它们已经受到关注，因为它们具有促进健康的益处和人类食用的安全性；因此，它们的使用，特别是作为疫苗和药物的传递剂，正在受到关注。益生菌菌株的定制不仅会改善有关这些菌株的益生菌潜力的数据，而且还会消除对这些益生菌的怀疑。本文重点介绍生物工程益生菌的方法，并讨论其治疗应用的潜在前景，包括免疫调节、认知健康和抗癌治疗。