Cadmium-tolerant (6 mM) Aspergillus niger (RCMB 002002) biomass was challenged with aqueous cadmium chloride (1 mM) followed by sodium sulfide (9 mM) at 37°C for 96 h under shaking conditions (200 rpm), resulting in the formation of highly stable polydispersed cadmium sulfide nanoparticles (CdSNPs). Scanning electron microscopy revealed the presence of spherical particles measuring approximately 5 nm. A light scattering detector (LSD) showed that 100% of the CSNPs measure from 2.7 to 7.5 nm. Structural analyses by both powder X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) confirmed the presence of cubic CdS nanoparticles (CdSNPs) capped with fungal proteins. These CdSNPs showed emission spectra with a broad fluorescence peak at 420 nm and UV absorption onset at 430 nm that shifted to 445 nm after three months of incubation. The CdSNPs showed antimicrobial activity against E. coli, Pseudomonas vulgaris, Staphylococcus aureus, and Bacillus subtilis, and no antimicrobial activity was detected against Candida albicans. The biosynthesized CdSNPs have cytotoxic activity, with 50% inhibitory concentrations (IC50) of 190 μg mL-1 against MCF7, 246 μg mL-1 against PC3, and 149 μg mL-1 against A549 cell lines.

中文翻译：

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黑曲霉对硫化镉纳米颗粒的抗癌和抗菌活性

耐镉 (6 mM) 黑曲霉 (RCMB 002002) 生物质依次用氯化镉水溶液 (1 mM) 和硫化钠 (9 mM) 在 37°C 振荡条件 (200 rpm) 下处理 96 小时，导致形成高度稳定的多分散硫化镉纳米颗粒（CdSNP）。扫描电子显微镜显示存在约 5 nm 的球形颗粒。光散射检测器 (LSD) 显示 100% 的 CSNP 测量范围为 2.7 到 7.5 nm。通过粉末 X 射线衍射 (XRD) 和傅里叶变换红外光谱 (FTIR) 进行的结构分析证实，存在覆盖有真菌蛋白的立方硫化镉纳米颗粒 (CdSNP)。这些 CdSNP 显示发射光谱，在 420 nm 处有一个宽荧光峰，在 430 nm 处开始吸收紫外光，经过三个月的孵育后转移到 445 nm。CdSNPs 显示出对大肠杆菌、普通假单胞菌、金黄色葡萄球菌和枯草芽孢杆菌的抗菌活性，并且未检测到对白色念珠菌的抗菌活性。生物合成的 CdSNP 具有细胞毒活性，对 MCF7 的 50% 抑制浓度 (IC50) 为 190 μg mL-1，对 PC3 的抑制浓度为 246 μg mL-1，对 A549 细胞系的抑制浓度为 149 μg mL-1。