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Purification, Preliminary Structural Characterization, and In Vitro Inhibitory Effect on Digestive Enzymes by β-Glucan from Qingke (Tibetan Hulless Barley)
Advances in Polymer Technology ( IF 3.1 ) Pub Date : 2020-05-19 , DOI: 10.1155/2020/2709536 Jialiang Hu 1 , Yue Wu 2 , Huifang Xie 3 , Wanyin Shi 2 , Zhiyuan Chen 4 , Dan Jiang 4 , Hui Hu 5 , Xiangwei Zheng 1 , Jian Xu 2 , Yuejun Yang 2 , Yuancai Liu 2
Advances in Polymer Technology ( IF 3.1 ) Pub Date : 2020-05-19 , DOI: 10.1155/2020/2709536 Jialiang Hu 1 , Yue Wu 2 , Huifang Xie 3 , Wanyin Shi 2 , Zhiyuan Chen 4 , Dan Jiang 4 , Hui Hu 5 , Xiangwei Zheng 1 , Jian Xu 2 , Yuejun Yang 2 , Yuancai Liu 2
Affiliation
Background and Objective. Qingke (Tibetan hulless barley, Hordeum vulgare L.) contains a high content of β-glucan among all the cereal varieties. Although β-glucan has multiple physiological functions, the physiological function of qingke β-glucan was few studied. In this study, the β-glucan was isolated, purified, determined the structural characterization, and measured the inhibitory activity to enzymes correlating blood sugar and lipid. Methods. β-Glucan was isolated from enzymatic aqueous extract of qingke by using deproteinization, decolorization, DEAE-52 column chromatography, and sepharose CL-4B agarose gel column chromatography. The structure of the β-glucan was determined using FT-IR and 13C-NMR spectra analysis, and molecular mass by use of HPSEC-dRI-LS. The kinematic viscosity was measured. The inhibitory effects of this β-glucan on four enzymes were investigated. Results. This β-glucan had a uniform molecular weight of 201,000 Da with β-(1⟶4) as the main chain and β-(1⟶3) as a side chain. The β-glucan presented a relatively strong inhibitory activity on α-glucosidase, moderate inhibition on invertase, and a weak inhibition on α-amylase, whereas it did not inhibit lipase. Conclusion. The study indicates that the enzymatic β-glucan from qingke has the potential as natural auxiliary hypoglycemic additives in functional medicine or foods.
更新日期：2020-05-19
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