Degradable biomaterials have emerged as a promising type of medical materials because of their unique advantages of biocompatibility, biodegradability and biosafety. Owing to their bioabsorbable and biocompatible properties, magnesium-based biomaterials are considered as ideal degradable medical implants. However, the rapid corrosion of magnesium-based materials not only limits their clinical application but also necessitates a more specific biological evaluation system and biosafety standard. In this study, extracts of pure Mg and its calcium alloy were prepared using different media based on ISO 10993:12; the Mg²⁺ concentration and osmolality of each extract were measured. The biocompatibility was investigated using the MTT assay and xCELLigence real-time cell analysis (RTCA). Cytotoxicity tests were conducted with L929, MG-63 and human umbilical vein endothelial cell lines. The results of the RTCA highly matched with those of the MTT assay and revealed the different dynamic modes of the cytotoxic process, which are related to the differences in the tested cell lines, Mg-based materials and dilution rates of extracts. This study provides an insight on the biocompatibility of biodegradable materials from the perspective of cytotoxic dynamics and suggests the applicability of RTCA for the cytotoxic evaluation of degradable biomaterials.

中文翻译：

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一种基于实时细胞分析的可降解生物材料动态生物相容性评估新方法。

可降解生物材料由于其生物相容性，生物降解性和生物安全性的独特优势，已成为一种有前途的医疗材料。由于其生物可吸收和生物相容性，镁基生物材料被认为是理想的可降解医疗植入物。然而，镁基材料的快速腐蚀不仅限制了它们的临床应用，而且还需要更具体的生物学评估系统和生物安全标准。在这项研究中，纯Mg及其钙合金的提取物是根据ISO 10993：12使用不同的介质制备的。镁²⁺测量每种提取物的浓度和重量摩尔渗透压浓度。使用MTT分析和xCELLigence实时细胞分析（RTCA）研究了生物相容性。用L929，MG-63和人脐静脉内皮细胞系进行了细胞毒性试验。RTCA的结果与MTT分析的结果高度匹配，并揭示了细胞毒性过程的不同动态模式，这与受试细胞系，基于Mg的材料和提取物稀释率的差异有关。这项研究从细胞毒性动力学的角度提供了可生物降解材料的生物相容性的见解，并建议RTCA在可降解生物材料的细胞毒性评估中的适用性。