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Metabolic profiling of glyphosate-resistant sourgrass (Digitaria insularis)
Weed Technology ( IF 1.3 ) Pub Date : 2020-03-05 , DOI: 10.1017/wet.2020.31
Tiago Gazola , Leandro Bianchi , Márcio Furriela Dias , Caio A. Carbonari , Edivaldo D. Velini

Putative glyphosate-resistant sourgrass was collected to determine its resistance level and to evaluate its metabolic profile after resistance. Although accumulation of shikimic acid is known to occur in glyphosate-susceptible populations, differences in the ability of resistant (R) and susceptible (S) biotypes to accumulate quinic acid, salicylic acid, and aminomethylphosphonic acid (AMPA) have been studied to a lesser extent. Our objective was to confirm glyphosate resistance in sourgrass and to understand the metabolic profile of these plants in response to the herbicide. Greenhouse experiments were carried out from January 2016 to June 2018. There were no significant differences in glyphosate translocation in the plants. No metabolism of glyphosate to AMPA was observed; therefore, metabolism of glyphosate to AMPA is not a mechanism in R biotypes. S biotypes showed higher concentrations of shikimic acid and quinic acid before glyphosate and accumulated less of both secondary acids in treated leaves 72 h after glyphosate application. Resistant biotypes showed higher concentrations of salicylic acid before glyphosate application.

中文翻译:

抗草甘膦酸草(Digitaria insularis)的代谢分析

收集假定的抗草甘膦酸草以确定其抗性水平并评估其抗性后的代谢特征。尽管已知莽草酸的积累发生在草甘膦易感人群中,但对抗性 (R) 和易感 (S) 生物型积累奎尼酸、水杨酸和氨甲基膦酸 (AMPA) 的能力差异进行了较少的研究。程度。我们的目标是确认酸草对草甘膦的抗性,并了解这些植物对除草剂的反应的代谢特征。温室实验于 2016 年 1 月至 2018 年 6 月进行。植物中草甘膦易位没有显着差异。没有观察到草甘膦代谢为 AMPA;所以,草甘膦代谢为 AMPA 不是 R 生物型的机制。S 生物型在草甘膦施用前显示出较高浓度的莽草酸和奎宁酸,而在施用草甘膦 72 小时后,处理过的叶片中两种次生酸的积累较少。抗性生物型在施用草甘膦之前表现出较高浓度的水杨酸。
更新日期:2020-03-05
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